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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ub-like Proteins

Anti-ISG15 antibody (ab227541)

Price and availability

278 083 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-ISG15 antibody (ab227541)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to ISG15
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-ISG15 antibody
    See all ISG15 primary antibodies
  • Description

    Rabbit polyclonal to ISG15
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human ISG15 (internal sequence). The exact sequence is proprietary.
    Database link: P05161

  • Positive control

    • WB: HeLa cell extract; Rat spleen extract. IHC-P: Human gastric cancer tissue. ICC/IF: HepG2 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ub-like Proteins
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • Ubiquitin like Modifiers

Images

  • Western blot - Anti-ISG15 antibody (ab227541)
    Western blot - Anti-ISG15 antibody (ab227541)
    All lanes : Anti-ISG15 antibody (ab227541) at 1/500 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : ISG15 Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 18 kDa

  • Western blot - Anti-ISG15 antibody (ab227541)
    Western blot - Anti-ISG15 antibody (ab227541)
    All lanes : Anti-ISG15 antibody (ab227541) at 1/500 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
    Lane 2 : ISG15 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 18 kDa



    15% SDS-PAGE gel.

  • Western blot - Anti-ISG15 antibody (ab227541)
    Western blot - Anti-ISG15 antibody (ab227541)
    Anti-ISG15 antibody (ab227541) at 1/2000 dilution + Rat spleen extract at 50 µg

    Predicted band size: 18 kDa



    15% SDS-PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISG15 antibody (ab227541)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISG15 antibody (ab227541)

    Paraffin-embedded human gastric cancer tissue stained for ISG15 using ab227541 at 1/500 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-ISG15 antibody (ab227541)
    Immunocytochemistry/ Immunofluorescence - Anti-ISG15 antibody (ab227541)

    4% paraformaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for ISG15 (green) using ab227541 at 1/100 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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