Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UFSP2 antibody [EPR13424] (ab185965)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13424] to UFSP2
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-UFSP2 antibody [EPR13424]
    See all UFSP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR13424] to UFSP2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal kidney, fetal heart, pancreas and HeLa cell lysates; HEK293T and HeLa cell lysates. IHC-P: Human stomach and kidney clear cell carcinoma tissues. ICC/IF: HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13424

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-UFSP2 antibody [EPR13424] (ab185965)
    Western blot - Anti-UFSP2 antibody [EPR13424] (ab185965)
    All lanes : Anti-UFSP2 antibody [EPR13424] (ab185965) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : UFSP2 knockout HEK293T cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab185965 observed at 53 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab185965 Anti-UFSP2 antibody [EPR13424]  was shown to specifically react with UFSP2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266368 (knockout cell lysate ab257782) was used. Wild-type and UFSP2 knockout samples were subjected to SDS-PAGE. ab185965 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-UFSP2 antibody [EPR13424] (ab185965)
    Immunocytochemistry/ Immunofluorescence - Anti-UFSP2 antibody [EPR13424] (ab185965)

    Immunofluorescent analysis of HeLa cells (-20℃ acetone-fixed) labeling UFSP2 with ab185965 at 1/100 dilution follwed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFSP2 antibody [EPR13424] (ab185965)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFSP2 antibody [EPR13424] (ab185965)

    Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling UFSP2 with ab185965 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-UFSP2 antibody [EPR13424] (ab185965)
    Western blot - Anti-UFSP2 antibody [EPR13424] (ab185965)
    All lanes : Anti-UFSP2 antibody [EPR13424] (ab185965) at 1/10000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human pancreas lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 53 kDa

  • Immunoprecipitation - Anti-UFSP2 antibody [EPR13424] (ab185965)
    Immunoprecipitation - Anti-UFSP2 antibody [EPR13424] (ab185965)

    Western blot analysis of immunoprecipitation pellet from Human fetal kidney lysate immunoprecipitated using ab185965 at 1/50 dilution (lane 1) or PBS control (lane 2).
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFSP2 antibody [EPR13424] (ab185965)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFSP2 antibody [EPR13424] (ab185965)

    Immunohistochemical analysis of paraffin-embedded Human kidney clear cell carcinoma tissue labeling UFSP2 with ab185965 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-UFSP2 antibody [EPR13424] (ab185965)
    Anti-UFSP2 antibody [EPR13424] (ab185965)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-UFSP2 antibody [EPR13424] (ab185965)

© 2021 Astana Biomed Group LLP. All rights reserved.