Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Anti-UFSP2 antibody [EPR13424] (ab185965)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13424] to UFSP2
Suitable for: WB, IHC-P, ICC/IF, IP
Knockout validated
Reacts with: Human

Product name

Anti-UFSP2 antibody [EPR13424]
See all UFSP2 primary antibodies
Description

Rabbit monoclonal [EPR13424] to UFSP2
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal kidney, fetal heart, pancreas and HeLa cell lysates; HEK293T and HeLa cell lysates. IHC-P: Human stomach and kidney clear cell carcinoma tissues. ICC/IF: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13424
Isotype

IgG
Research areas

Western blot - Anti-UFSP2 antibody [EPR13424] (ab185965)

All lanes : Anti-UFSP2 antibody [EPR13424] (ab185965) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : UFSP2 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 53 kDa
Observed band size: 53 kDa

Lanes 1-3: Merged signal (red and green). Green - ab185965 observed at 53 kDa. Red - loading control ab8245 observed at 36 kDa.

ab185965 Anti-UFSP2 antibody [EPR13424] was shown to specifically react with UFSP2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266368 (knockout cell lysate ab257782) was used. Wild-type and UFSP2 knockout samples were subjected to SDS-PAGE. ab185965 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-UFSP2 antibody [EPR13424] (ab185965)

Immunofluorescent analysis of HeLa cells (-20℃ acetone-fixed) labeling UFSP2 with ab185965 at 1/100 dilution follwed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFSP2 antibody [EPR13424] (ab185965)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling UFSP2 with ab185965 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-UFSP2 antibody [EPR13424] (ab185965)

All lanes : Anti-UFSP2 antibody [EPR13424] (ab185965) at 1/10000 dilution

Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human pancreas lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 53 kDa
Immunoprecipitation - Anti-UFSP2 antibody [EPR13424] (ab185965)

Western blot analysis of immunoprecipitation pellet from Human fetal kidney lysate immunoprecipitated using ab185965 at 1/50 dilution (lane 1) or PBS control (lane 2).
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFSP2 antibody [EPR13424] (ab185965)

Immunohistochemical analysis of paraffin-embedded Human kidney clear cell carcinoma tissue labeling UFSP2 with ab185965 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-UFSP2 antibody [EPR13424] (ab185965)

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