Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AMPS antibody [EPR10746(B)] (ab154872)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10746(B)] to AMPS
  • Suitable for: WB, IP, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-AMPS antibody [EPR10746(B)]
    See all AMPS primary antibodies

  • Description

    Rabbit monoclonal [EPR10746(B)] to AMPS

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Mouse
    WB
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human AMPS.
    Database link: P30566

  • Positive control

    • WB: C6, Raw 264.7, NIH/3T3, HeLa, HepG2 and Jurkat whole cell lysate (ab7899). ICC/IF: HeLa cells. IP: NIH/3T3

  • General notes

     

     

    Previously labelled as Adenylosuccinate Lyase. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-AMPS antibody [EPR10746(B)] (ab154872)
    Western blot - Anti-AMPS antibody [EPR10746(B)] (ab154872)
    All lanes : Anti-AMPS antibody [EPR10746(B)] (ab154872) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw 264.7 cell lysate
    Lane 3 : NIH/3T3 cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : HepG2 cell lysate
    Lane 6 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 55 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-AMPS antibody [EPR10746(B)] (ab154872)
    Immunocytochemistry/ Immunofluorescence - Anti-AMPS antibody [EPR10746(B)] (ab154872)

    Immunofluorescence analysis of HeLa cells labeling AMPS with ab154872 at 1/250 dilution.

  • Immunoprecipitation - Anti-AMPS antibody [EPR10746(B)] (ab154872)
    Immunoprecipitation - Anti-AMPS antibody [EPR10746(B)] (ab154872)

    AMPS was immunoprecipitated from 1mg of NIH/3T3 (Mouse embyro fibroblast cells) whole cell extract with ab154872 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab154872 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution.

    Lane 1: NIH/3T3 whole cell extract, 10 µg (Input).

    Lane 2: ab154872 IP in NIH/3T3 whole cell extract.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab154872 in NIH/3T3 whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 seconds

     

  • Anti-AMPS antibody [EPR10746(B)] (ab154872)
    Anti-AMPS antibody [EPR10746(B)] (ab154872)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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