Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NDUFB9 antibody [EPR15955] (ab188581)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15955] to NDUFB9
  • Suitable for: IHC-P, WB, Flow Cyt, ICC/IF, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-NDUFB9 antibody [EPR15955]
    See all NDUFB9 primary antibodies

  • Description

    Rabbit monoclonal [EPR15955] to NDUFB9

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal liver, HeLa, 293 and Jurkat lysates. IHC-P: Human kidney tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15955

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-NDUFB9 antibody [EPR15955] (ab188581)
    Western blot - Anti-NDUFB9 antibody [EPR15955] (ab188581)
    All lanes : Anti-NDUFB9 antibody [EPR15955] (ab188581) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : NDUFB9 knockout HeLa cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 21 kDa
    Observed band size: 22 kDa
    why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab188581 observed at 22 kDa. Red - loading control ab8245 observed at 36 kDa.

    ab188581 Anti-NDUFB9 antibody [EPR15955] was shown to specifically react with NDUFB9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265946 (knockout cell lysate ab258065) was used. Wild-type and NDUFB9 knockout samples were subjected to SDS-PAGE. ab188581 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB9 antibody [EPR15955] (ab188581)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB9 antibody [EPR15955] (ab188581)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFB9 with ab188581 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-NDUFB9 antibody [EPR15955] (ab188581)
    Immunocytochemistry/ Immunofluorescence - Anti-NDUFB9 antibody [EPR15955] (ab188581)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed 293 cells labeling NDUFB9 with ab188581 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi.

  • Flow Cytometry - Anti-NDUFB9 antibody [EPR15955] (ab188581)
    Flow Cytometry - Anti-NDUFB9 antibody [EPR15955] (ab188581)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling NDUFB9 with ab188581 at 1/440 dilution compared to a Rabbit monoclonal IgG isotype control, followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Western blot - Anti-NDUFB9 antibody [EPR15955] (ab188581)
    Western blot - Anti-NDUFB9 antibody [EPR15955] (ab188581)
    All lanes : Anti-NDUFB9 antibody [EPR15955] (ab188581) at 1/10000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 22 kDa why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-NDUFB9 antibody [EPR15955] (ab188581)
    Immunoprecipitation - Anti-NDUFB9 antibody [EPR15955] (ab188581)

    Western blot analysis of NDUFB9 immunoprecipitated from Human fetal liver lysate using ab188581 at 1/50 (Lane 1). Lane 2: PBS instead of Human fetal liver lysates.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Anti-NDUFB9 antibody [EPR15955] (ab188581)
    Anti-NDUFB9 antibody [EPR15955] (ab188581)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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