Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Wednesday March 10, 2021

Anti-NDUFB9 antibody [EPR15955] (ab188581)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15955] to NDUFB9
Suitable for: IHC-P, WB, Flow Cyt, ICC/IF, IP
Knockout validated
Reacts with: Human

Product name

Anti-NDUFB9 antibody [EPR15955]
See all NDUFB9 primary antibodies
Description

Rabbit monoclonal [EPR15955] to NDUFB9
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal liver, HeLa, 293 and Jurkat lysates. IHC-P: Human kidney tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15955
Isotype

IgG
Research areas

Western blot - Anti-NDUFB9 antibody [EPR15955] (ab188581)

All lanes : Anti-NDUFB9 antibody [EPR15955] (ab188581) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : NDUFB9 knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 21 kDa
Observed band size: 22 kDa
why is the actual band size different from the predicted?

Lanes 1-3: Merged signal (red and green). Green - ab188581 observed at 22 kDa. Red - loading control ab8245 observed at 36 kDa.

ab188581 Anti-NDUFB9 antibody [EPR15955] was shown to specifically react with NDUFB9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265946 (knockout cell lysate ab258065) was used. Wild-type and NDUFB9 knockout samples were subjected to SDS-PAGE. ab188581 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB9 antibody [EPR15955] (ab188581)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFB9 with ab188581 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-NDUFB9 antibody [EPR15955] (ab188581)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 293 cells labeling NDUFB9 with ab188581 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi.
Flow Cytometry - Anti-NDUFB9 antibody [EPR15955] (ab188581)

Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling NDUFB9 with ab188581 at 1/440 dilution compared to a Rabbit monoclonal IgG isotype control, followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Western blot - Anti-NDUFB9 antibody [EPR15955] (ab188581)

All lanes : Anti-NDUFB9 antibody [EPR15955] (ab188581) at 1/10000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293 cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 21 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Immunoprecipitation - Anti-NDUFB9 antibody [EPR15955] (ab188581)

Western blot analysis of NDUFB9 immunoprecipitated from Human fetal liver lysate using ab188581 at 1/50 (Lane 1). Lane 2: PBS instead of Human fetal liver lysates.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Anti-NDUFB9 antibody [EPR15955] (ab188581)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com