Human SLC31A1 (CTR1) knockout HEK293T cell pellet (ab279190)
Overview
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Product name
Human SLC31A1 (CTR1) knockout HEK293T cell pellet
See all SLC31A1 / CTR1 kits -
Product overview
Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.
Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation. -
Parental Cell Line
HEK293T -
Organism
Human -
Mutation description
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2. -
Passage number
Knockout validation
Sanger SequencingNotes
Pellet size: 5 million cells/vial.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human SLC31A1 knockout HEK293T cell pellet 1 vial Human wild-type HEK293T cell pellet 1 vial -
Research areas
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Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Target
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Function
High-affinity, saturable copper transporter involved in dietary copper uptake. -
Sequence similarities
Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily. -
Post-translational
modificationsO-Glycosylation at Thr-27 protects from proteolytic cleavage in the N-terminal extracellular domain. -
Cellular localization
Cell membrane. Localizes to the apical membrane in intestinal epithelial cells. - Information by UniProt
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Alternative names
- Copper transport 1 homolog
- Copper transporter 1
- COPT1
see all
Properties
-
Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human SLC31A1 knockout HEK293T cell pellet 1 vial Human wild-type HEK293T cell pellet 1 vial -
Research areas
-
Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Images
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Sanger Sequencing - Human SLC31A1 (CTR1) knockout HEK293T cell pellet (ab279190)Homozygous: 1 bp insertion in exon2
