Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human CD85d Antibody Pair - BSA and Azide free (ab270387)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

Overview

  • Product name

    Human CD85d Antibody Pair - BSA and Azide free
    See all ILT-4 kits

  • Assay type

    ELISA set

  • Range

    62.5 pg/ml - 4000 pg/ml

  • Species reactivity

    Reacts with: Human

  • Product overview

    The Antibody Pair can be used to quantify Human CD85d. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab267804), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.

  • Carrier free

    Yes
  • Components 10 x 96 tests
    Human CD85d Capture Antibody (unconjugated) 1 x 100µg
    Human CD85d Detector Antibody (unconjugated) 1 x 100µg

  • Research areas

  • Function

    Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Involved in the down-regulation of the immune response and the development of tolerance. Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.

  • Tissue specificity

    Expressed on monocytes and B-cells, and at lower levels on dendritic cells. Detected at low levels in natural killer (NK) cells.

  • Sequence similarities

    Contains 4 Ig-like C2-type (immunoglobulin-like) domains.

  • Domain

    Contains 3 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

  • Post-translational
    modifications

    Phosphorylated on tyrosine residues. Dephosphorylated by PTPN6.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Alternative names

    • CD85 antigen-like family member D
    • CD85d
    • CD85d antigen
    • ILT 4
    • ILT-4
    • ILT4
    • Immunoglobulin like transcript 4
    • Immunoglobulin-like transcript 4
    • Leukocyte immunoglobulin like receptor 2
    • Leukocyte immunoglobulin like receptor subfamily B member 2
    • Leukocyte immunoglobulin-like receptor 2
    • Leukocyte immunoglobulin-like receptor subfamily B member 2
    • Leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 2
    • LILRA6
    • LILRB2
    • LIR 2
    • LIR-2
    • LIR2
    • LIRb
    • LIRB2_HUMAN
    • MIR 10
    • MIR-10
    • MIR10
    • Monocyte/macrophage immunoglobulin like receptor 10
    • Monocyte/macrophage immunoglobulin-like receptor 10
    see all

  • Database links

    Images

    • Sandwich ELISA - Human CD85d Antibody Pair - BSA and Azide free (ab270387)
      Sandwich ELISA - Human CD85d Antibody Pair - BSA and Azide free (ab270387)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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