Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AKR1B10 antibody [EPR14421] - BSA and Azide free (ab232623)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14421] to AKR1B10 - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-AKR1B10 antibody [EPR14421] - BSA and Azide free
    See all AKR1B10 primary antibodies

  • Description

    Rabbit monoclonal [EPR14421] to AKR1B10 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human stomach tissue.

  • General notes

    Ab232623 is the carrier-free version of ab192865. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232623 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1B10 antibody [EPR14421] - BSA and Azide free (ab232623)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1B10 antibody [EPR14421] - BSA and Azide free (ab232623)

    Immunohistochemical analysis of paraffin embedded Human liver tissue labeling AKR1B10 with ab192865 at a 1/1000 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192865).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-AKR1B10 antibody [EPR14421] - BSA and Azide free (ab232623)
    Immunocytochemistry/ Immunofluorescence - Anti-AKR1B10 antibody [EPR14421] - BSA and Azide free (ab232623)

    Immunofluorescent analysis of 4% paraformaldehyde fixed SW480 cells labeling AKR1B10 with ab192865 at a 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary antibody at a 1/400 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192865).

  • Flow Cytometry - Anti-AKR1B10 antibody [EPR14421] - BSA and Azide free (ab232623)
    Flow Cytometry - Anti-AKR1B10 antibody [EPR14421] - BSA and Azide free (ab232623)

    Flow cytometric analysis of A549 cells labeling AKR1B10 using ab192865 at a 1/50 dilution (red). Goat anti rabbit IgG (FITC) was used as the secondary antibody at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (black). Unlabeled control cells without incubation with primary antibody and secondary antibody (blue). Cells were fixed in 2% paraformaldehyde.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192865).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1B10 antibody [EPR14421] - BSA and Azide free (ab232623)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1B10 antibody [EPR14421] - BSA and Azide free (ab232623)

    Immunohistochemical analysis of paraffin embedded human stomach tissue sections labeling AKR1B10 using ab192865 at a 1/1,000 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192865).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-AKR1B10 antibody [EPR14421] - BSA and Azide free (ab232623)
    Anti-AKR1B10 antibody [EPR14421] - BSA and Azide free (ab232623)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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