Anti-AKR1B10 antibody [EPR14421] (ab192865)
![Anti-AKR1B10 antibody [EPR14421] (ab192865)](https://www.abcam.com/ps/products/192/ab192865/Images/ab192865-230667-anti-akr1b10-antibody-epr14421-western-blot.jpg "Anti-AKR1B10 antibody [EPR14421] (ab192865)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14421] to AKR1B10
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-AKR1B10 antibody [EPR14421]
See all AKR1B10 primary antibodies -
Description
Rabbit monoclonal [EPR14421] to AKR1B10 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- A549 and SW480 cell lysate; Human liver and stomach tissues; SW480 and A549 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14421 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AKR1B10 antibody [EPR14421] (ab192865)Anti-AKR1B10 antibody [EPR14421] (ab192865) at 1/10000 dilution + A549 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDaBlocking buffer and concentration: 5% NFDM/TBST
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![Western blot - Anti-AKR1B10 antibody [EPR14421] (ab192865)](https://www.abcam.com/ps/products/192/ab192865/Images/ab192865-230666-anti-akr1b10-antibody-epr14421-western-blot.jpg)
Western blot - Anti-AKR1B10 antibody [EPR14421] (ab192865)Anti-AKR1B10 antibody [EPR14421] (ab192865) at 1/1000 dilution + SW480 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDaBlocking buffer and concentration: 5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1B10 antibody [EPR14421] (ab192865)](https://www.abcam.com/ps/products/192/ab192865/Images/ab192865-230665-anti-akr1b10-antibody-epr14421-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1B10 antibody [EPR14421] (ab192865)Immunohistochemical analysis of paraffin embedded Human liver tissue labeling AKR1B10 with ab192865 at a 1/1000 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1B10 antibody [EPR14421] (ab192865)](https://www.abcam.com/ps/products/192/ab192865/Images/ab192865-230664-anti-akr1b10-antibody-epr14421-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1B10 antibody [EPR14421] (ab192865)Immunohistochemical analysis of paraffin embedded human stomach tissue sections labeling AKR1B10 using ab192865 at a 1/1,000 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-AKR1B10 antibody [EPR14421] (ab192865)](https://www.abcam.com/ps/products/192/ab192865/Images/ab192865-230663-anti-akr1b10-antibody-epr14421-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-AKR1B10 antibody [EPR14421] (ab192865)Immunofluorescent analysis of 4% paraformaldehyde fixed SW480 cells labeling AKR1B10 with ab192865 at a 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary antibody at a 1/400 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100.
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![Flow Cytometry - Anti-AKR1B10 antibody [EPR14421] (ab192865)](https://www.abcam.com/ps/products/192/ab192865/Images/ab192865-230662-anti-akr1b10-antibody-epr14421-flow-cytometry.jpg)
Flow Cytometry - Anti-AKR1B10 antibody [EPR14421] (ab192865)Flow cytometric analysis of A549 cells labeling AKR1B10 using ab192865 at a 1/50 dilution (red). Goat anti rabbit IgG (FITC) was used as the secondary antibody at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (black). Unlabeled control cells without incubation with primary antibody and secondary antibody (blue). Cells were fixed in 2% paraformaldehyde.
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![Anti-AKR1B10 antibody [EPR14421] (ab192865)](https://www.abcam.com/ps/products/192/ab192865/Images/ab192865-7-benefits-of-recombinant-antibodies.png)
Anti-AKR1B10 antibody [EPR14421] (ab192865)
