Anti-PDHA1 antibody (ab97352)
Key features and details
- Rabbit polyclonal to PDHA1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PDHA1 antibody
See all PDHA1 primary antibodies -
Description
Rabbit polyclonal to PDHA1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant fragment within Human PDHA1 aa 39-295. The exact sequence is proprietary. (NP_000275).
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Positive control
- WB: 293T, A431, H1299, HeLa S3, HepG2, MOLT4 and Raji ICC/IF: HeLa cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PDHA1 antibody (ab97352)All lanes : Anti-PDHA1 antibody (ab97352) at 1/1000 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : Pyruvate Dehydrogenase E1-alpha subunit Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 43 kDa
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Western blot - Anti-PDHA1 antibody (ab97352)All lanes : Anti-PDHA1 antibody (ab97352) at 1/1000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : MOLT4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 43 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody (ab97352)ab97352, at a 1/200 dilution, staining PDHA1 in paraformaldehyde fixed HeLa cells by Immunofluorescence. Lower image was merged with DNA probe.
