Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µg 328 339 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Mouse monoclonal [9H9AF5] to PDHA1
Suitable for: WB, ICC/IF, Flow Cyt
Knockout validated
Reacts with: Mouse, Rat, Cow, Human
Isotype: IgG1

Product name

Anti-PDHA1 antibody [9H9AF5]
See all PDHA1 primary antibodies
Description

Mouse monoclonal [9H9AF5] to PDHA1
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein. This information is considered to be commercially sensitive.
Positive control
- Isolated mitochondria from Human, Bovine, Rat and Mouse hearts, HepG2 lysate; cultured, normal Human embryonic lung fibroblasts (strain MRC5); HL60 cells.
General notes

This antibody clone is manufactured by Abcam.

Product was previously marketed under the MitoSciences sub-brand.

If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline
Concentration information loading...
Purity

IgG fraction
Purification notes

ab110330 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality

Monoclonal
Clone number

9H9AF5
Isotype

IgG1
Light chain type

kappa
Research areas

Western blot - Anti-PDHA1 antibody [9H9AF5] (ab110330)

All lanes : Anti-PDHA1 antibody [9H9AF5] (ab110330) at 1 µg/ml

Lane 1 : Wild-type HeLa whole cell lysate
Lane 2 : PDHA1 knockout HeLa whole cell lysate
Lane 3 : HEK-293 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 43 kDa
Observed band size: 43 kDa

Lanes 1 - 3: Merged signal (red and green). Green - ab110330 observed at 43 kDa. Red - loading control, ab52866, observed at 50 kDa.

ab110330 was shown to specifically react with PDHA1 in wild-type HeLa cells as signal was lost in PDHA1 knockout cells. Wild-type and PDHA1 knockout samples were subjected to SDS-PAGE. Ab110330 and ab52866 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 1 ug/ml and 1/1000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye^®680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-PDHA1 antibody [9H9AF5] (ab110330)

All lanes : Anti-PDHA1 antibody [9H9AF5] (ab110330) at 1 µg/ml

Lane 1 : Isolated mitochondria from Human heart at 10 µg
Lane 2 : Isolated mitochondria from Bovine heart at 4 µg
Lane 3 : Isolated mitochondria from Rat heart at 10 µg
Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
Lane 5 : hepG2 cell lysate at 20 µg

Predicted band size: 43 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody [9H9AF5] (ab110330)

Immunocytochemistry analysis using ab110330 at 5µg/ml staining PDHA1 in cultured, normal Human embryonic lung fibroblasts (strain MRC5) followed by AlexaFluor® 488 goat anti-mouse IgG1 secondary antibody (2 µg/ml).
Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody [9H9AF5] (ab110330) 'This image is courtesy of an Abreview submitted by George Allen'

Immunocytochemical analysis of SH-SY5Y human neuroblastoma labeling PDHA1 with ab110330 at 1/500 dilution.
See Abreview
Flow Cytometry - Anti-PDHA1 antibody [9H9AF5] (ab110330)

Flow cytometric analysis using ab110330 at 1µg/ml staining PDHA1 in HL60 cells (blue). Isotype control antibody (red).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com