HRP Anti-PDHA1 antibody [8D10E6] (ab197962)
![HRP Anti-PDHA1 antibody [8D10E6] (ab197962)](https://www.abcam.com/ps/products/197/ab197962/Images/ab197962-242624-WBab1979621.jpg "HRP Anti-PDHA1 antibody [8D10E6] (ab197962)")
Key features and details
- HRP Mouse monoclonal [8D10E6] to PDHA1
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-PDHA1 antibody [8D10E6]
See all PDHA1 primary antibodies -
Description
HRP Mouse monoclonal [8D10E6] to PDHA1 -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Full length native protein (purified) corresponding to Human PDHA1.
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Positive control
- WB: Human heart mitochondrial lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
ab110334 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
8D10E6 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - HRP Anti-PDHA1 antibody [8D10E6] (ab197962)HRP Anti-PDHA1 antibody [8D10E6] (ab197962) at 1/5000 dilution + Human heart tissue lysate - mitochondrial extract (ab110337) at 5 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab197962 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
