Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11098] to PDHA1 - BSA and Azide free
  • Suitable for: Flow Cyt, IP, ICC/IF, IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PDHA1 antibody [EPR11098] - BSA and Azide free
    See all PDHA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11098] to PDHA1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab170730)

  • General notes

    Ab176835 is the carrier-free version of ab168379. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab176835 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PDHA1 with unpurified ab168379 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168379).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling PDHA1 with unpurified ab168379 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168379).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)
    Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)

    Immunofluorescent analysis of HepG2 cells labeling PDHA1 with unpurified ab168379 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168379).

  • Immunoprecipitation - Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)
    Immunoprecipitation - Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)

    ab168379 (purified) at 1:20 dilution (2ug) immunoprecipitating PDHA1 in HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
    Lane 2 (+): ab168379 & HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab168379 in HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168379).

  • Flow Cytometry - Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)
    Flow Cytometry - Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)

    Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling PDHA1 with purified ab168379 at 1:40 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168379).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)

    Immunocytochemistry/ Immunofluorescence analysis of HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labeling PDHA1 with Purified ab168379 at 1:100 dilution (3.5μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168379).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling PDHA1 with Purified ab168379 at 1:200 dilution (1.76 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168379).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling PDHA1 with Purified ab168379 at 1:200 dilution (1.76 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168379).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling PDHA1 with Purified ab168379 at 1:200 dilution (1.76 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168379).

  • Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)
    Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)

    Immunocytochemistry/Immunofluorescence analysis Jurkat (human acute T cell leukemia) labelling PDHA1 with purified ab168379 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    Control: PBS only

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168379).

  • Flow Cytometry - Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)
    Flow Cytometry - Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)

    Flow cytometric analysis of permeabilized Jurkat cells labeling PDHA1 (red) with unpurified ab168379 at 1/10 dilution, or a rabbit IgG (negative) (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168379).

  • Immunoprecipitation - Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)
    Immunoprecipitation - Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)

    Detection of PDHA1 by Western Blot of Immunprecipitate. 293T cell lysate immunoprecipitated using unpurified ab168379 at 1/10 dilution; HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168379).

  • Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)
    Anti-PDHA1 antibody [EPR11098] - BSA and Azide free (ab176835)

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