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Signal Transduction Protein Trafficking Vesicle Transport Regulation

Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] - BSA and Azide free (ab173371)

Price and availability

526 012 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] - BSA and Azide free (ab173371)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR654(2)A] to eIF4EBP1 (phospho T70) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] - BSA and Azide free
    See all eIF4EBP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR654(2)A] to eIF4EBP1 (phospho T70) - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody detects eIF4EBP1 phosphorylated at threonine 70.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab173371 is the carrier-free version of ab75831 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab173371 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR654(2)A
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • RNAi
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Eukaryotic Initiation factors (eIF's)
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Metabolic disorders

Images

  • Western blot - Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] - BSA and Azide free (ab173371)
    Western blot - Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] - BSA and Azide free (ab173371)
    All lanes : Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] (ab75831) at 1/500 dilution

    Lane 1 : 293T cell lysates un-treated
    Lane 2 : 293T cell lysates treated with FBS

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 13 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab75831, the same antibody clone in a different buffer formulation.

     

  • Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] - BSA and Azide free (ab173371)
    Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] - BSA and Azide free (ab173371)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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