Anti-ART3 antibody [EPR7220(2)] - BSA and Azide free (ab251155)
![Anti-ART3 antibody [EPR7220(2)] - BSA and Azide free (ab251155)](https://www.abcam.com/ps/products/251/ab251155/Images/ab251155-3-anti-art3-antibody-epr7220-2-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg "Anti-ART3 antibody [EPR7220(2)] - BSA and Azide free (ab251155)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7220(2)] to ART3 - BSA and Azide free
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-ART3 antibody [EPR7220(2)] - BSA and Azide free
See all ART3 primary antibodies -
Description
Rabbit monoclonal [EPR7220(2)] to ART3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251155 is the carrier-free version of ab192870. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251155 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR7220(2) -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ART3 antibody [EPR7220(2)] - BSA and Azide free (ab251155)This data was developed using ab192870, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling ART3 using ab192870 at 1/500 dilution (0.6 μg/ml). A prediluted HRP Polymer for Rabbit IgG was used as secondary. Counterstain: Hematoxylin. Negative control also shown. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-ART3 antibody [EPR7220(2)] - BSA and Azide free (ab251155)](https://www.abcam.com/ps/products/251/ab251155/Images/ab251155-403360-anti-art3-antibody-epr7220-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ART3 antibody [EPR7220(2)] - BSA and Azide free (ab251155)Anti-ART3 antibody [EPR7220(2)] - C-terminal (ab192870) at 1/10000 dilution + Human testis tissue lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDaThis data was developed using ab192870, the same antibody clone in a different buffer formulation.
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![Immunoprecipitation - Anti-ART3 antibody [EPR7220(2)] - BSA and Azide free (ab251155)](https://www.abcam.com/ps/products/251/ab251155/Images/ab251155-1-anti-art3-antibody-epr7220-2-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ART3 antibody [EPR7220(2)] - BSA and Azide free (ab251155)This data was developed using ab192870, the same antibody clone in a different buffer formulation.Immunoprecipitation analysis of Human testis lysate labeling ART3 using ab192870 at 1/20 dilution (Lane 1). PBS negative control (Lane 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as the secondary antibodyNote: The band observed at 41 kDa may be due to the removal of a 27 amino acid propeptide (mature form). -
![Anti-ART3 antibody [EPR7220(2)] - BSA and Azide free (ab251155)](https://www.abcam.com/ps/products/251/ab251155/Images/ab251155-4-benefits-of-recombinant-antibodies.png)
Anti-ART3 antibody [EPR7220(2)] - BSA and Azide free (ab251155)