Anti-HOOK3 antibody (ab237529)
Key features and details
- Rabbit polyclonal to HOOK3
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HOOK3 antibody
See all HOOK3 primary antibodies -
Description
Rabbit polyclonal to HOOK3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Xenopus laevis
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Immunogen
Recombinant fragment corresponding to Human HOOK3 aa 350-500.
Database link: Q86VS8 -
Positive control
- WB: HEK-293 whole cell lysate. IHC-P: Human colon cancer tissue. IP: HEK-293 whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity greater than 95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK3 antibody (ab237529)
Paraffin-embedded human colon cancer tissue stained for HOOK3 with ab237529 at a 1/300 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Western blot - Anti-HOOK3 antibody (ab237529)Anti-HOOK3 antibody (ab237529) at 1/500 dilution + HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
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Immunoprecipitation - Anti-HOOK3 antibody (ab237529)HOOK3 was immunoprecipitated from HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate using 6 µg of ab237529.
Lane 1: Rabbit control IgG in HEK-293 whole cell lysate.
Lane 2: ab237529 (6 µg) in HEK-293 whole cell lysate.
Lane 3: HEK-293 whole cell lysate (20 µg input).
For western blotting an HRP-conjugated Protein G antibody was used as the secondary antibody at a 1/2000 dilution.
