Anti-Dynamin 2 antibody [EPR9053(2)(ABC)] (ab151555)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9053(2)(ABC)] to Dynamin 2
- Suitable for: WB, IHC-P, ICC
- Reacts with: Human
Overview
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Product name
Anti-Dynamin 2 antibody [EPR9053(2)(ABC)]
See all Dynamin 2 primary antibodies -
Description
Rabbit monoclonal [EPR9053(2)(ABC)] to Dynamin 2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human Dynamin 2 aa 750-850. The exact sequence is proprietary.
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Positive control
- HeLa, K562, Jurkat and fetal brain lysates; Human kidney and skeletal muscle tissues.K-562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9053(2)(ABC) -
Isotype
IgG -
Research areas
Images
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized K-562 cells labelling Dynamin 2 with ab151555 at 1/50 (19.56 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in K-562 cell line ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2 ug/ml dilution.
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All lanes : Anti-Dynamin 2 antibody [EPR9053(2)(ABC)] (ab151555) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Fetal brain lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 98 kDa
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Dynamin 2 with ab151555 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labelling Dynamin 2 with ab151555 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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