Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LSM5 antibody [EPR10690] (ab184568)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10690] to LSM5
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-LSM5 antibody [EPR10690]
    See all LSM5 primary antibodies

  • Description

    Rabbit monoclonal [EPR10690] to LSM5

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • T47-D lysate, Human astrocytoma or stomach tissue, T47-D cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-LSM5 antibody [EPR10690] (ab184568)
    Flow Cytometry - Anti-LSM5 antibody [EPR10690] (ab184568)

    Flow cytometry analysis of T47-D cells using ab184568 at a 1/110 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-LSM5 antibody [EPR10690] (ab184568)
    Immunocytochemistry/ Immunofluorescence - Anti-LSM5 antibody [EPR10690] (ab184568)

    Immunofluorescence analysis of 4% paraformaldehyde fixed T47-D cells labeling LSM5 with ab184568 at a 1/500 dilution. Goat anti rabbit IgG (Alexa Fluor®555) secondary used at a 1/200 diution. Conterstained with Dapi (right image).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10690] (ab184568)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10690] (ab184568)

    Immunohistochemical analysis of paraffin embedded Human stomach tissue labeling LSM5 with ab184568 a 1/100 dilution. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10690] (ab184568)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10690] (ab184568)

    Immunohistochemical analysis of paraffin embedded Human astrocytoma tissue labeling LSM5 with ab184568 at a 1/100 dilution. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-LSM5 antibody [EPR10690] (ab184568)
    Western blot - Anti-LSM5 antibody [EPR10690] (ab184568)
    Anti-LSM5 antibody [EPR10690] (ab184568) at 1/10000 dilution + T47-D lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 10 kDa

  • Anti-LSM5 antibody [EPR10690] (ab184568)
    Anti-LSM5 antibody [EPR10690] (ab184568)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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