Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DGKA antibody [EPR23176-101] (ab243647)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23176-101] to DGKA
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-DGKA antibody [EPR23176-101]
    See all DGKA primary antibodies

  • Description

    Rabbit monoclonal [EPR23176-101] to DGKA

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293, HeLa and Jurkat whole cell lysates; Human tonsil lysate. IHC-P: Human tonsil and Human spleen tissues. IP: Jurkat whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23176-101

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKA antibody [EPR23176-101] (ab243647)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKA antibody [EPR23176-101] (ab243647)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling DGKA with ab243647 at 1/4000 dilution (0.163 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on T cells of human tonsil is observed. The section was incubated with ab243647 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Western blot - Anti-DGKA antibody [EPR23176-101] (ab243647)
    Western blot - Anti-DGKA antibody [EPR23176-101] (ab243647)
    All lanes : Anti-DGKA antibody [EPR23176-101] (ab243647) at 1/1000 dilution

    Lane 1 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : Jurkat (human t cell leukemia t lymphocyte) whole cell lysate
    Lane 4 : Human tonsil lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
    Lane 4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 83 kDa
    Observed band size: 83 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes1-2:125 seconds; Lanes 3-4: 26 seconds.

    The expression profile / molecular weight observed is consistent with what has been described in the literature (PMID: 22048771).

  • Immunoprecipitation - Anti-DGKA antibody [EPR23176-101] (ab243647)
    Immunoprecipitation - Anti-DGKA antibody [EPR23176-101] (ab243647)

    DGKA was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte) whole cell lysate with ab243647 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab243647 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate 10ug

    Lane 2: ab243647 IP in Jurkat whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab243647 in Jurkat whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 40 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKA antibody [EPR23176-101] (ab243647)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKA antibody [EPR23176-101] (ab243647)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling DGKA with ab243647 at 1/4000 dilution (0.163 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human spleen. The section was incubated with ab243647 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Anti-DGKA antibody [EPR23176-101] (ab243647)
    Anti-DGKA antibody [EPR23176-101] (ab243647)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-DGKA antibody [EPR23176-101] (ab243647)

© 2021 Astana Biomed Group LLP. All rights reserved.