Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

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Anti-DGKA antibody [EPR23176-101] (ab243647)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23176-101] to DGKA
Suitable for: WB, IHC-P, IP
Reacts with: Human

Product name

Anti-DGKA antibody [EPR23176-101]
See all DGKA primary antibodies
Description

Rabbit monoclonal [EPR23176-101] to DGKA
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293, HeLa and Jurkat whole cell lysates; Human tonsil lysate. IHC-P: Human tonsil and Human spleen tissues. IP: Jurkat whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23176-101
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKA antibody [EPR23176-101] (ab243647)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling DGKA with ab243647 at 1/4000 dilution (0.163 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on T cells of human tonsil is observed. The section was incubated with ab243647 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Western blot - Anti-DGKA antibody [EPR23176-101] (ab243647)

All lanes : Anti-DGKA antibody [EPR23176-101] (ab243647) at 1/1000 dilution

Lane 1 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Jurkat (human t cell leukemia t lymphocyte) whole cell lysate
Lane 4 : Human tonsil lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Lane 4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 83 kDa
Observed band size: 83 kDa

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lanes1-2:125 seconds; Lanes 3-4: 26 seconds.

The expression profile / molecular weight observed is consistent with what has been described in the literature (PMID: 22048771).
Immunoprecipitation - Anti-DGKA antibody [EPR23176-101] (ab243647)

DGKA was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte) whole cell lysate with ab243647 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab243647 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate 10ug

Lane 2: ab243647 IP in Jurkat whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab243647 in Jurkat whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 40 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGKA antibody [EPR23176-101] (ab243647)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling DGKA with ab243647 at 1/4000 dilution (0.163 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human spleen. The section was incubated with ab243647 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Anti-DGKA antibody [EPR23176-101] (ab243647)

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