Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸ 10 µl 113 913 ₸

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Anti-IL-33 antibody [EPR20417] (ab207737)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20417] to IL-33
Suitable for: WB, IHC-P
Reacts with: Rat, Human

Product name

Anti-IL-33 antibody [EPR20417]
See all IL-33 primary antibodies
Description

Rabbit monoclonal [EPR20417] to IL-33
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Rat

Human

WB
Rat

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human IL33 recombinant protein. THP-1 treated with Phorbol-12-myristate-13-acetate and LPS lysate. Human artery, lung, tonsil, stomach and colon lysate. His-tagged rat IL33 recombinant protein. Rat spleen, kidney and lung lysate. IHC-P: Rat lung and spleen tissue. Human tonsil tissue and kidney cancer tissue. ICC/IF: THP-1 cells.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Rat Human
WB	Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20417
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR20417] (ab207737)

Immunohistochemical analysis of paraffin embedded rat spleen tissue labeling IL33 with ab207737 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on endothelium cells of rat spleen (PMID: 12819012, PMID: 18836528) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-IL-33 antibody [EPR20417] (ab207737)

Anti-IL-33 antibody [EPR20417] (ab207737) at 1/1000 dilution + human IL33 recombinant protein (aa112-270), 10 ng

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 30 kDa
Observed band size: 18 kDa
why is the actual band size different from the predicted?

Exposure time: 2 seconds

Blocking: 5% NFDM/TBST
Western blot - Anti-IL-33 antibody [EPR20417] (ab207737)

All lanes : Anti-IL-33 antibody [EPR20417] (ab207737) at 1/1000 dilution

Lane 1 : Untreated THP-1 (human monocytic leukemia monocyte), whole cell lysate
Lane 2 : THP-1 treated with 50 nM Phorbol-12-myristate-13-acetate (PMA, ab120297) and 5 ug/ml lipopolysaccharides (LPS) for 24 hours, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 30 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?

Exposure time: 10 seconds

The level of IL33 expression can be induced by PMA and LPS treatment (PMID: 19559631).

Blocking : 5% NFDM/TBST
Western blot - Anti-IL-33 antibody [EPR20417] (ab207737)

All lanes : Anti-IL-33 antibody [EPR20417] (ab207737) at 1/1000 dilution

Lane 1 : Human artery
Lane 2 : Human lung
Lane 3 : Human tonsil
Lane 4 : Human stomach
Lane 5 : Human colon

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 30 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?

Blocking: 5% NFDM/TBST

Exposure times:

Lanes 1-3,5: 3 minutes

Lanes 4: 30 seconds
Western blot - Anti-IL-33 antibody [EPR20417] (ab207737)

Anti-IL-33 antibody [EPR20417] (ab207737) at 1/1000 dilution + Recombinant Rat IL-33 protein (ab207126)

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 30 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes
Western blot - Anti-IL-33 antibody [EPR20417] (ab207737)

All lanes : Anti-IL-33 antibody [EPR20417] (ab207737) at 1/1000 dilution

Lane 1 : Rat spleen
Lane 2 : Rat kidney
Lane 3 : Rat lung

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 30 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?

Blocking: 5% NFDM/TBST

Exposure times:

Lane 1: 3 seconds

Lane 2: 10 seconds

Lane3: 5 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR20417] (ab207737)

Immunohistochemical analysis of paraffin embedded rat lung tissue labeling IL33 with ab207737 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on epithelium and endothelium cells of rat lung (PMID: 18836528) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR20417] (ab207737)

Immunohistochemical analysis of paraffin embedded human tonsil tissue labeling IL33 with ab207737 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on high endothelial venules of human tonsil (PMID: 12819012) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR20417] (ab207737)

Immunohistochemical analysis of paraffin embedded human kidney cancer tissue labeling IL33 with ab207737 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on endothelial cells of human kidney cancer (PMID: 12819012) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-IL-33 antibody [EPR20417] (ab207737)

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