Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Selenium Binding Protein 1/SBP antibody (ab90135)

Key features and details

  • Rabbit polyclonal to Selenium Binding Protein 1/SBP
  • Suitable for: WB, ICC/IF, IP, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Selenium Binding Protein 1/SBP antibody
    See all Selenium Binding Protein 1/SBP primary antibodies

  • Description

    Rabbit polyclonal to Selenium Binding Protein 1/SBP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Human Selenium Binding Protein 1/SBP aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab90251)

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Human Liver: Human Colon; Human Spleen; Mouse Liver; Mouse Lung; Rat Liver.

  • General notes

    Previously labelled as Selenium Binding Protein 1. 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab90135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IP Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    Selenium-binding protein which may be involved in the sensing of reactive xenobiotics in the cytoplasm. May be involved in intra-Golgi protein transport.

  • Tissue specificity

    Highly expressed in liver, lung, colon, prostate, kidney and pancreas. In brain, present both in neurons and glia (at protein level). Down-regulated in lung adenocarcinoma, colorectal carcinoma and ovarian cancer. Two-fold up-regulated in brain and blood from schizophrenia patients.

  • Sequence similarities

    Belongs to the selenium-binding protein family.

  • Post-translational
    modifications

    Phosphorylated.
    The N-terminus is blocked.

  • Cellular localization

    Nucleus. Cytoplasm > cytosol. Membrane. May associate with Golgi membrane. May associate with the membrane of autophagosomes.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 56 kDa selenium binding protein antibody
    • 56 kDa selenium-binding protein antibody
    • hSBP antibody
    • LPSB antibody
    • SBP antibody
    • SBP1 antibody
    • SBP1_HUMAN antibody
    • SBP56 antibody
    • Selenbp1 antibody
    • Selenbp2 antibody
    • Selenium Binding Protein 1 antibody
    • Selenium binding protein 2 antibody
    • Selenium binding protein1 antibody
    • Selenium-binding protein 1 antibody
    • SELNBP1 antibody
    • SP56 antibody
    see all

Images

  • Western blot - Anti-Selenium Binding Protein 1/SBP antibody (ab90135)
    Western blot - Anti-Selenium Binding Protein 1/SBP antibody (ab90135)
    All lanes : Anti-Selenium Binding Protein 1/SBP antibody (ab90135) at 1/500 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : SELENBP1 knockout HeLa cell lysate
    Lane 3 : HT-29 cell lysate
    Lane 4 : SW 480 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-3 : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
    Lane 4 : ab216773 at 1/10000 dilution

    Predicted band size: 52 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab90135 observed at 60 kDa. Red - loading control ab8245 observed at 36 kDa.

    ab90135 Anti-Selenium Binding Protein 1/SBP antibody was shown to specifically react with Selenium Binding Protein 1/SBP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265279 (knockout cell lysate ab257662) was used. Wild-type and Selenium Binding Protein 1/SBP knockout samples were subjected to SDS-PAGE. ab90135 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-Selenium Binding Protein 1/SBP antibody (ab90135)
    Western blot - Anti-Selenium Binding Protein 1/SBP antibody (ab90135)
    All lanes : Anti-Selenium Binding Protein 1/SBP antibody (ab90135) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human colon tissue lysate - total protein (ab30051)
    Lane 3 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 52 kDa
    Observed band size: 52 kDa
    Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute
  • Immunocytochemistry/ Immunofluorescence - Anti-Selenium Binding Protein 1/SBP antibody (ab90135)
    Immunocytochemistry/ Immunofluorescence - Anti-Selenium Binding Protein 1/SBP antibody (ab90135)
    ICC/IF image of ab90135 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab90135, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 5µg/ml, and in 100% Methanol fixed (5 min) HeLa, and Hek293 cells at 5µg/ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Selenium Binding Protein 1/SBP antibody (ab90135)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Selenium Binding Protein 1/SBP antibody (ab90135)
    IHC image of ab90135 staining in Normal Human Liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab90135, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Western blot - Anti-Selenium Binding Protein 1/SBP antibody (ab90135)
    Western blot - Anti-Selenium Binding Protein 1/SBP antibody (ab90135)
    All lanes : Anti-Selenium Binding Protein 1/SBP antibody (ab90135) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Lung (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 52 kDa
    Observed band size: 52 kDa


    Exposure time: 1 minute
  • Immunoprecipitation - Anti-Selenium Binding Protein 1/SBP antibody (ab90135)
    Immunoprecipitation - Anti-Selenium Binding Protein 1/SBP antibody (ab90135)

    Selenium Binding Protein 1/SBP was immunoprecipitated using 0.5mg Mouse Liver tissue lysate, 5µg of Rabbit polyclonal to Selenium Binding Protein 1/SBP and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab90135.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 52kDa; Selenium Binding Protein 1/SBP

References (3)

Publishing research using ab90135? Please let us know so that we can cite the reference in this datasheet.

ab90135 has been referenced in 3 publications.

  • Caswell DR  et al. Tumor Suppressor Activity of Selenbp1, a Direct Nkx2-1 Target, in Lung Adenocarcinoma. Mol Cancer Res 16:1737-1749 (2018). PubMed: 30002193
  • Chen F  et al. Selenium-binding protein 1 in head and neck cancer is low-expression and associates with the prognosis of nasopharyngeal carcinoma. Medicine (Baltimore) 95:e4592 (2016). IHC-P ; Human . PubMed: 27583873
  • Song H  et al. Distribution and inhibition effect of Seleno-L-Methionine on 4T1 mouse mammary carcinoma. Int J Physiol Pathophysiol Pharmacol 7:76-86 (2015). Mouse . PubMed: 26330897

Images

  • Western blot - Anti-Selenium Binding Protein 1/SBP antibody (ab90135)
    Western blot - Anti-Selenium Binding Protein 1/SBP antibody (ab90135)
    All lanes : Anti-Selenium Binding Protein 1/SBP antibody (ab90135) at 1/500 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : SELENBP1 knockout HeLa cell lysate
    Lane 3 : HT-29 cell lysate
    Lane 4 : SW 480 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-3 : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
    Lane 4 : ab216773 at 1/10000 dilution

    Predicted band size: 52 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab90135 observed at 60 kDa. Red - loading control ab8245 observed at 36 kDa.

    ab90135 Anti-Selenium Binding Protein 1/SBP antibody was shown to specifically react with Selenium Binding Protein 1/SBP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265279 (knockout cell lysate ab257662) was used. Wild-type and Selenium Binding Protein 1/SBP knockout samples were subjected to SDS-PAGE. ab90135 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-Selenium Binding Protein 1/SBP antibody (ab90135)
    Western blot - Anti-Selenium Binding Protein 1/SBP antibody (ab90135)
    All lanes : Anti-Selenium Binding Protein 1/SBP antibody (ab90135) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human colon tissue lysate - total protein (ab30051)
    Lane 3 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 52 kDa
    Observed band size: 52 kDa
    Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute
  • Immunocytochemistry/ Immunofluorescence - Anti-Selenium Binding Protein 1/SBP antibody (ab90135)
    Immunocytochemistry/ Immunofluorescence - Anti-Selenium Binding Protein 1/SBP antibody (ab90135)
    ICC/IF image of ab90135 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab90135, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 5µg/ml, and in 100% Methanol fixed (5 min) HeLa, and Hek293 cells at 5µg/ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Selenium Binding Protein 1/SBP antibody (ab90135)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Selenium Binding Protein 1/SBP antibody (ab90135)
    IHC image of ab90135 staining in Normal Human Liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab90135, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Western blot - Anti-Selenium Binding Protein 1/SBP antibody (ab90135)
    Western blot - Anti-Selenium Binding Protein 1/SBP antibody (ab90135)
    All lanes : Anti-Selenium Binding Protein 1/SBP antibody (ab90135) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Lung (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 52 kDa
    Observed band size: 52 kDa


    Exposure time: 1 minute
  • Immunoprecipitation - Anti-Selenium Binding Protein 1/SBP antibody (ab90135)
    Immunoprecipitation - Anti-Selenium Binding Protein 1/SBP antibody (ab90135)

    Selenium Binding Protein 1/SBP was immunoprecipitated using 0.5mg Mouse Liver tissue lysate, 5µg of Rabbit polyclonal to Selenium Binding Protein 1/SBP and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab90135.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 52kDa; Selenium Binding Protein 1/SBP

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