Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BST2/Tetherin antibody [EPR5648] (ab134061)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5648] to BST2/Tetherin
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-BST2/Tetherin antibody [EPR5648]
    See all BST2/Tetherin primary antibodies

  • Description

    Rabbit monoclonal [EPR5648] to BST2/Tetherin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human BST2/Tetherin aa 100-200 (extracellular).
    Database link: Q10589

  • Positive control

    • WB: A431, U937, Molt-4, Human spleen and HeLa lysates; Human kidney tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as BST2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
    Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
    All lanes : Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution

    Lane 1 : Wild-type A431 cell lysate
    Lane 2 : BST2 knockout A431 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : SH-SY5Y cell lysate

    Lysates/proteins at 40 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 20 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    ab134061 was shown to react with BST2 in A431 wild-type cells in western blot. Loss of signal was observed when BST2 knockout sample was used. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab134061 overnight at 4°C at a 1 in 1000 dilution and ab184095 (Mouse Anti-GAPDH antibody [mAbcam 9484] - Alexa Fluor® 680) at a 1 in 1000 dilution. Blots were incubated with HRP conjugated Goat anti-Rabbit (H+L) secondary antibody at 1/5000 for 1 hour at room temperature before development with Optiblot ECL reagent (ab133456) and imaging.

  • Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
    Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
    Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 20 kDa
    Observed band size: 28-40 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
    Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
    Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution (Purified) + Human fetal spleen whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 20 kDa
    Observed band size: 30 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Diluting buffer and concentration 5% NFDM/TBST

    This antibody detects a non-specific band at 65 KDa.

    This image was produced using unpurified antibody.

  • Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
    Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
    All lanes : Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution

    Lane 1 : U937 lysate
    Lane 2 : Molt-4 lysate
    Lane 3 : Human spleen lysate
    Lane 4 : HeLa lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 20 kDa



    This image was produced using unpurified antibody.

  • Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
    Anti-BST2/Tetherin antibody [EPR5648] (ab134061)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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