Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BST2/Tetherin antibody [EPR20202-150] (ab243230)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20202-150] to BST2/Tetherin
  • Suitable for: ICC/IF, IP, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-BST2/Tetherin antibody [EPR20202-150]
    See all BST2/Tetherin primary antibodies

  • Description

    Rabbit monoclonal [EPR20202-150] to BST2/Tetherin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa whole cell lysate in the loading buffer containing DTT; K562 whole cell lysate in the loading buffer containing DTT. U937 whole cell lysate. ICC/IF: HeLa and U-937 cells. Flow Cyt: HeLa cells. IP: HeLa whole cell lysate.

  • General notes

     This product was previously labelled as BST2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR20202-150] (ab243230)
    Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR20202-150] (ab243230)

    BST2/Tetherin was immunoprecipitated from 0.35 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab243230 at 1/40 dilution. Western blot was perfromed on the immunoprecipitate using ab243230 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used afor detection at 1/5000 dilution.

    Lane 1: HeLa whole cell lysate 10 µg (input)
    Lane 2: ab243230 IP in HeLa whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab243230 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST
    Exposure time: 3 seconds.

  • Flow Cytometry - Anti-BST2/Tetherin antibody [EPR20202-150] (ab243230)
    Flow Cytometry - Anti-BST2/Tetherin antibody [EPR20202-150] (ab243230)

    Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling BST2/Tetherin with ab243230 at 1/700 dilution (Red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR20202-150] (ab243230)
    Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR20202-150] (ab243230)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-937 (human histiocytic lymphoma cell line) cells labeling BST2/Tetherin with ab243230 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody (Green). Confocal image showing cytoplasmic staining in U-937 cells (PMID: 20529266).

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR20202-150] (ab243230)
    Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR20202-150] (ab243230)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling BST2/Tetherin with ab243230 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody (Green). Confocal image showing cytoplasmic staining in HeLa cells (PMID: 20529266).

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-BST2/Tetherin antibody [EPR20202-150] (ab243230)
    Western blot - Anti-BST2/Tetherin antibody [EPR20202-150] (ab243230)
    Anti-BST2/Tetherin antibody [EPR20202-150] (ab243230) at 1/1000 dilution + U-937 (human histiocytic lymphoma cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 20 kDa


    Exposure time: 125 seconds


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

     

  • Western blot - Anti-BST2/Tetherin antibody [EPR20202-150] (ab243230)
    Western blot - Anti-BST2/Tetherin antibody [EPR20202-150] (ab243230)
    All lanes : Anti-BST2/Tetherin antibody [EPR20202-150] (ab243230) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate in the loading buffer containing DTT
    Lane 2 : HeLa whole cell lysate in the loading buffer without DTT
    Lane 3 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate in the loading buffer containing
    Lane 4 : K562 whole cell lysate in the loading buffer without DTT

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 20 kDa
    Observed band size: 35,70 kDa
    why is the actual band size different from the predicted?


    Exposure time: 48 seconds


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 19196977; PMID: 19737401).

    BST2/Tetherin is a glycosylated protein, its calculated MW is 20 kDa, and the observed MW is 35 kDa, which is consistent with the literature.

    Both 35 and 70 kDa bands were detected under the reducing condition, whereas under the non-reducing condition, only the 70 kDa band was detected.

     

  • Anti-BST2/Tetherin antibody [EPR20202-150] (ab243230)
    Anti-BST2/Tetherin antibody [EPR20202-150] (ab243230)

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