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Anti-BST2/Tetherin antibody [EPR20202-150] - BSA and Azide free (ab243561)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20202-150] to BST2/Tetherin - BSA and Azide free
  • Suitable for: WB, ICC/IF, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-BST2/Tetherin antibody [EPR20202-150] - BSA and Azide free
    See all BST2/Tetherin primary antibodies

  • Description

    Rabbit monoclonal [EPR20202-150] to BST2/Tetherin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa and U-937 cells. Flow Cyt: HeLa cells. IP: HeLa whole cell lysate.

  • General notes

    ab243561 is the carrier-free version of ab243230 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab243561 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as BST2

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR20202-150] - BSA and Azide free (ab243561)
    Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR20202-150] - BSA and Azide free (ab243561)

    BST2/Tetherin was immunoprecipitated from 0.35 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab243230 at 1/40 dilution. Western blot was perfromed on the immunoprecipitate using ab243230 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

    Lane 1: HeLa whole cell lysate 10 µg (input)
    Lane 2: ab243230 IP in HeLa whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab243230 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST
    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243230).

  • Flow Cytometry - Anti-BST2/Tetherin antibody [EPR20202-150] - BSA and Azide free (ab243561)
    Flow Cytometry - Anti-BST2/Tetherin antibody [EPR20202-150] - BSA and Azide free (ab243561)

    Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling BST2/Tetherin with ab243230 at 1/700 dilution (Red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243230).

  • Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR20202-150] - BSA and Azide free (ab243561)
    Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR20202-150] - BSA and Azide free (ab243561)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-937 (human histiocytic lymphoma cell line) cells labeling BST2/Tetherin with ab243230 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody (Green). Confocal image showing cytoplasmic staining in U-937 cells (PMID: 20529266).

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243230).

  • Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR20202-150] - BSA and Azide free (ab243561)
    Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR20202-150] - BSA and Azide free (ab243561)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling BST2/Tetherin with ab243230 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody (Green). Confocal image showing cytoplasmic staining in HeLa cells (PMID: 20529266).

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243230).

  • Anti-BST2/Tetherin antibody [EPR20202-150] - BSA and Azide free (ab243561)
    Anti-BST2/Tetherin antibody [EPR20202-150] - BSA and Azide free (ab243561)

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