Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (ab40755)
![Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (ab40755)](https://www.abcam.com/ps/products/40/ab40755/Images/ab40755-255514-anti-pi-3-kinase-p85-alpha-antibody-ep380y-western-blot.jpg "Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (ab40755)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP380Y] to PI 3 Kinase p85 alpha
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-PI 3 Kinase p85 alpha antibody [EP380Y]
See all PI 3 Kinase p85 alpha primary antibodies -
Description
Rabbit monoclonal [EP380Y] to PI 3 Kinase p85 alpha -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human PI 3 Kinase p85 alpha aa 700-800 (C terminal). The exact sequence is proprietary.
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Positive control
- A431, MCF7 or Raji cells lysate, HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP380Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (ab40755)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PI 3 Kinase p85 alpha knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lane 4: THP1 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab40755 observed at 90 kDa. Red - loading control, ab181602, observed at 37 kDa.ab40755 was shown to recognize PI 3 Kinase p85 alpha in wild-type HAP1 cells along with additional cross-reactive bands. No Band was observed when PI 3 Kinase p85 alpha knockout samples were examined. Wild-type and PI 3 Kinase p85 alpha knockout samples were subjected to SDS-PAGE. ab40755 and ab181602 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (ab40755)](https://www.abcam.com/ps/products/40/ab40755/Images/ab40755-53859-anti-pi-3-kinase-p85-alpha-antibody-ep380y-western-blot.jpg)
Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (ab40755)All lanes : Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (ab40755) at 1/2000 dilution
Lane 1 : A431 cells
Lane 2 : MCF-7 cells
Lane 3 : Raji cells
Lysates/proteins at 10 µg per lane.
Predicted band size: 85 kDa
Observed band size: 85 kDa
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![Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (ab40755)](https://www.abcam.com/ps/products/40/ab40755/Images/ab40755-5-benefits-of-recombinant-antibodies.png)
Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (ab40755)
