Anti-5-hydroxymethylcytosine (5-hmC) antibody (ab231902)
Key features and details
- Rabbit polyclonal to 5-hydroxymethylcytosine (5-hmC)
- Suitable for: IP, Dot blot
- Isotype: IgG
Overview
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Product name
Anti-5-hydroxymethylcytosine (5-hmC) antibody
See all 5-hydroxymethylcytosine (5-hmC) primary antibodies -
Description
Rabbit polyclonal to 5-hydroxymethylcytosine (5-hmC) -
Host species
Rabbit -
Tested applications
Suitable for: IP, Dot blotmore details -
Immunogen
Chemical/ Small Molecule corresponding to 5-hydroxymethylcytosine (5-hmC) conjugated to keyhole limpet haemocyanin.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-5-hydroxymethylcytosine (5-hmC) antibody (ab231902)
An hydroxymethylated DNA IP (hMeDIP) was performed using ab231902.
IgG isotype antibodies from rabbit were used as negative controls. DNA fragments of 300-500 bp were used. 1 µg of Hela (Human epithelial cell line from cervix adenocarcinoma) cells DNA were spiked with non-methylated, methylated, and hydroxymethylated fragments. The IP’d material has been analysed by qPCR using the primer pair specifc for the 3 different control sequences.
Lane 1: Unmethylated control.
Lane 2: Methylated control.
Lane 3: Hydroxymethylated control.
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Dot Blot - Anti-5-hydroxymethylcytosine (5-hmC) antibody (ab231902)Dot Blot analysis of ab231902 with the C, mC and hmC PCR controls.
100 to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the hmC, mC and C PCR controls were spotted on a membrane, then incubated with ab231902 at a 1:200 dilution.
Exposure time: 30 seconds.
