Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713)
![Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713)](https://www.abcam.com/ps/products/279/ab279713/Images/ab279713-453162-AntiDopamine-Receptor-D1-EPR24102105-WB.jpg "Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24102-105] to Dopamine Receptor D1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-Dopamine Receptor D1 antibody [EPR24102-105]
See all Dopamine Receptor D1 primary antibodies -
Description
Rabbit monoclonal [EPR24102-105] to Dopamine Receptor D1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatWB MouseRat
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse striatum; Rat striatum. IHC-P: Mouse brain and corpus striatum; Rat corpus striatum.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24102-105 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713)All lanes : Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713) at 1/1000 dilution
Lane 1 : Mouse striatum tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Rat striatum tissue lysate
Lane 5 : Rat heart tissue lysate
Lane 6 : Rat kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 49 kDa
Observed band size: 95-100 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: Lane 1-3: 15 seconds Lane 4-6: 69 seconds.
The molecular weight observed is consistent with what have been described in literature (PMID: 28316027).
Negative control: heart, kidney (PMID: 2144334)
Samples are non-boiled as boiling may cause protein aggregates. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713)](https://www.abcam.com/ps/products/279/ab279713/Images/ab279713-453163-AntiDopamine-Receptor-D1-EPR24102105-IHC-mouse-brain.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713)Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling Dopamine Receptor D1 with ab279713 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining is mainly on the corpus striatum of mouse brain. The section was incubated with ab279713 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713)](https://www.abcam.com/ps/products/279/ab279713/Images/ab279713-453164-AntiDopamine-Receptor-D1-EPR24102105-IHC-mouse-corpus-striatum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713)Immunohistochemical analysis of paraffin-embedded Mouse corpus striatum tissue labeling Dopamine Receptor D1 with ab279713 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the corpus striatum of mouse brain. The section was incubated with ab279713 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713)](https://www.abcam.com/ps/products/279/ab279713/Images/ab279713-453165-AntiDopamine-Receptor-D1-EPR24102105-IHC-rat-corpus-striatum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713)Immunohistochemical analysis of paraffin-embedded Rat corpus striatum tissue labeling Dopamine Receptor D1 with ab279713 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat corpus striatum of mouse brain. The section was incubated with ab279713 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins