Antibodies, Proteins, Kits and Reagents for Life Science

Anti-PI 3 Kinase p85 alpha antibody [6G10] (ab135253)

Key features and details

Mouse monoclonal [6G10] to PI 3 Kinase p85 alpha
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Mouse, Human
Isotype: IgG1

Product name

Anti-PI 3 Kinase p85 alpha antibody [6G10]
See all PI 3 Kinase p85 alpha primary antibodies
Description

Mouse monoclonal [6G10] to PI 3 Kinase p85 alpha
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC/IF	Human
WB	Mouse

See all applications and species data

Immunogen

Purified recombinant fragment of Human PI 3 Kinase p85 alpha (amino acids 159-388) expressed in E.coli

Run BLAST with Run BLAST with
Positive control
- Human PI 3 Kinase p85 alpha recombinant protein; HeLa, HepG2 and NIH3T3 cells; cervical cancer and rectum cancer tissues.
General notes

This product was changed from ascites to supernatant. Lot no’s high than GR130547-5 are from Tissue Culture Supernatant

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: 99% PBS

Protein stabilizer at 0.5%.
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

6G10
Isotype

IgG1
Research areas

Western blot - Anti-PI 3 Kinase p85 alpha antibody [6G10] (ab135253)

Anti-PI 3 Kinase p85 alpha antibody [6G10] (ab135253) at 1/500 dilution + Human PI 3 Kinase p85 alpha recombinant protein.

Predicted band size: 83.6 kDa

Note: the predicted band size of the full length protein is 83.6 kDa. The expected band size of the recombinant fragment used here is 53.4 kDa.
Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [6G10] (ab135253)

Immunofluorescence analysis of HeLa cells using ab135253 at 1/100 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [6G10] (ab135253)

Immunofluorescence analysis of HepG2 cells using ab135253 at 1/100 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [6G10] (ab135253)

Flow cytometric analysis of NIH3T3 cells using ab135253 at 1/200 dilution (green) and negative control (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [6G10] (ab135253)

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ab135253 at 1/200 dilution with DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [6G10] (ab135253)

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ab135253 at 1/200 dilution with DAB staining.

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