Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PI 3 Kinase p85 alpha antibody [6G10] (ab135253)

Key features and details

  • Mouse monoclonal [6G10] to PI 3 Kinase p85 alpha
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-PI 3 Kinase p85 alpha antibody [6G10]
    See all PI 3 Kinase p85 alpha primary antibodies

  • Description

    Mouse monoclonal [6G10] to PI 3 Kinase p85 alpha

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Human
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Purified recombinant fragment of Human PI 3 Kinase p85 alpha (amino acids 159-388) expressed in E.coli

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human PI 3 Kinase p85 alpha recombinant protein; HeLa, HepG2 and NIH3T3 cells; cervical cancer and rectum cancer tissues.

  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR130547-5 are from Tissue Culture Supernatant

Properties

Images

  • Western blot - Anti-PI 3 Kinase p85 alpha antibody [6G10] (ab135253)
    Western blot - Anti-PI 3 Kinase p85 alpha antibody [6G10] (ab135253)
    Anti-PI 3 Kinase p85 alpha antibody [6G10] (ab135253) at 1/500 dilution + Human PI 3 Kinase p85 alpha recombinant protein.

    Predicted band size: 83.6 kDa



    Note: the predicted band size of the full length protein is 83.6 kDa. The expected band size of the recombinant fragment used here is 53.4 kDa.

  • Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [6G10] (ab135253)
    Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [6G10] (ab135253)
    Immunofluorescence analysis of HeLa cells using ab135253 at 1/100 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [6G10] (ab135253)
    Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [6G10] (ab135253)
    Immunofluorescence analysis of HepG2 cells using ab135253 at 1/100 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [6G10] (ab135253)
    Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [6G10] (ab135253)
    Flow cytometric analysis of NIH3T3 cells using ab135253 at 1/200 dilution (green) and negative control (red).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [6G10] (ab135253)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [6G10] (ab135253)
    Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ab135253 at 1/200 dilution with DAB staining.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [6G10] (ab135253)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [6G10] (ab135253)
    Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ab135253 at 1/200 dilution with DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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