Human PIK3R1 (PI 3 Kinase p85 alpha) knockout HeLa cell lysate (ab257029)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab261938 - Human PIK3R1 knockout HeLa cell lysate 1 x 100µg ab255929 - Human wild-type HeLa cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10
Images
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Western blot - Human PIK3R1 (PI 3 Kinase p85 alpha) knockout HeLa cell lysate (ab257029)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: PIK3R1 knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab133595 observed at 90 kDa. Red - loading control ab8245 observed at 37 kDa.
ab133595 Recombinant Anti-PI 3 Kinase p85 alpha antibody [EPR5513] was shown to specifically react with PI 3 Kinase p85 alpha in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265116 (knockout cell lysate ab257029) was used. Wild-type and PI 3 Kinase p85 alpha knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab133595 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Western blot - Human PIK3R1 (PI 3 Kinase p85 alpha) knockout HeLa cell lysate (ab257029)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: PIK3R1 knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab191606 observed at 90 kDa. Red - loading control ab8245 observed at 37 kDa.
ab191606 Anti-PI 3 Kinase p85 alpha antibody [EPR18702] was shown to specifically react with PI 3 Kinase p85 alpha in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265116 (knockout cell lysate ab257029) was used. Wild-type and PI 3 Kinase p85 alpha knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab191606 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human PIK3R1 knockout HeLa cell lysate (ab257029)Allele-1: Insertion of the selection cassette in exon10
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Sanger Sequencing - Human PIK3R1 knockout HeLa cell lysate (ab257029)Allele-2: Insertion of the selection cassette in exon10