Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] - BSA and Azide free (ab238170)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19852] to IP6K1 (phospho S118 + S121) - BSA and Azide free
  • Suitable for: WB, Dot blot, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] - BSA and Azide free
    See all IP6K1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19852] to IP6K1 (phospho S118 + S121) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with a His-tagged human IP6K1 (WT) expression vector, whole cell lysate.

  • General notes

    Ab238170 is the carrier-free version of ab214816. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238170 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] - BSA and Azide free (ab238170)
    Western blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] - BSA and Azide free (ab238170)
    All lanes : Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816) at 1/10000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a His-tagged human IP6K1 (WT) expression vector, whole cell lysate
    Lane 2 : HEK-293T transfected with a His-tagged human IP6K1 (WT) expression vector, whole cell lysate. The WB membrane was treated with alkaline phosphatase for 1h at 37°C
    Lane 3 : HEK-293T transfected with a His-tagged human IP6K1 S118A+S121A mutant expression vector, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 50 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?


    Exposure time: 26 seconds


    Blocking/diluting buffer: 5% NFDM/TBST.

    The band at approximately 30 kDa likely represents degradation fragments of IP6K1 (pS118 and pS121) protein.

    The plasmids were kindly provided by our collaborator Dr. Feng Rao NIBS.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214816).

  • Immunoprecipitation - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] - BSA and Azide free (ab238170)
    Immunoprecipitation - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] - BSA and Azide free (ab238170)

    IP6K1 (phospho S118 + S121) was immunoprecipitated from 0.35 mg MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate with ab214816 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab214816 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.


    Lane 1: MOLT-4 whole cell lysate 10 µg (Input).
    Lane 2: ab214816 IP in MOLT-4 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab214816 in MOLT-4 whole cell lysate (-).

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 minute.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214816).

  • Dot Blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] - BSA and Azide free (ab238170)
    Dot Blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] - BSA and Azide free (ab238170)

    Dot blot analysis of IP6K1 (phospho S118 + S121) labeled with ab214816 at 1/1000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody was used at 1/100,000 dilution..

    Lane 1: IP6K1 (phospho S118 + S121) peptide (aa114-125).

    Lane 2: IP6K1 (phospho S118 + S121) peptide (aa116-127).

    Lane 3: IP6K1 (phospho S118) peptide (aa114-127).

    Lane 4: IP6K1 (phospho S121) peptide (aa114-127).

    Lane 5: IP6K1 non-phospho peptide (aa114-127).

    Exposure time: 58 seconds.

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214816).

  • Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] - BSA and Azide free (ab238170)
    Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] - BSA and Azide free (ab238170)

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