Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19852] to IP6K1 (phospho S118 + S121)
  • Suitable for: WB, Dot blot, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852]
    See all IP6K1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19852] to IP6K1 (phospho S118 + S121)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Dot Blot: IP6K1 (phospho S118 + S121) peptide (aa114-125) and IP6K1 (phospho S118) peptide (aa114-127). WB: HEK-293T transfected with His-tagged human IP6K1 (WT) expression vector, whole cell lysate; MOLT-4 whole cell lysate. IP: MOLT-4 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunoprecipitation - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816)
    Immunoprecipitation - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816)

    IP6K1 (phospho S118 + S121) was immunoprecipitated from 0.35 mg MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate with ab214816 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab214816 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5,000 dilution


    Lane 1: MOLT-4 whole cell lysate 10 µg (Input).
    Lane 2: ab214816 IP in MOLT-4 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab214816 in MOLT-4 whole cell lysate (-).

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 minute.

  • Western blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816)
    Western blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816)
    All lanes : Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816) at 1/5000 dilution

    Lane 1 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
    Lane 2 : MOLT-4 whole cell lysate. The WB membrane was treated with alkaline phosphatase for 1h at 37°C

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 50 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?


    Exposure time: 26 seconds


    Blocking/diluting buffer: 5% NFDM/TBST.

    The bands migrating at approximately 100 kDa are likely to be non-specific.

  • Western blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816)
    Western blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816)
    All lanes : Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816) at 1/10000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a His-tagged human IP6K1 (WT) expression vector, whole cell lysate
    Lane 2 : HEK-293T transfected with a His-tagged human IP6K1 (WT) expression vector whole cell lysate. The WB membrane was treated with alkaline phosphatase for 1h at 37°C
    Lane 3 : HEK-293T transfected with a His-tagged human IP6K1 S118A+S121A mutant expression vector, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 50 kDa


    Exposure time: 26 seconds


    Blocking/diluting buffer: 5% NFDM/TBST.

    The band at approximately 30 kDa likely represents degradation fragments of IP6K1 (pS118 and pS121) protein.

    The plasmids were kindly provided by our collaborator Dr. Feng Rao NIBS.

  • Dot Blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816)
    Dot Blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816)

    Dot blot analysis of IP6K1 (phospho S118 + S121) labeled with ab214816 at 1/1000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody was used at 1/100,000 dilution..

    Lane 1: IP6K1 (phospho S118 + S121) peptide (aa114-125).

    Lane 2: IP6K1 (phospho S118 + S121) peptide (aa116-127).

    Lane 3: IP6K1 (phospho S118) peptide (aa114-127).

    Lane 4: IP6K1 (phospho S121) peptide (aa114-127).

    Lane 5: IP6K1 non-phospho peptide (aa114-127).

    Exposure time: 58 seconds.

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

  • Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816)
    Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816)

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