Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ROR gamma antibody [EPR20006] - BSA and Azide free (ab232516)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20006] to ROR gamma - BSA and Azide free
  • Suitable for: IHC-P, WB, IP, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-ROR gamma antibody [EPR20006] - BSA and Azide free
    See all ROR gamma primary antibodies

  • Description

    Rabbit monoclonal [EPR20006] to ROR gamma - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Our data suggests that this antibody is specific to ROR gamma isoform 2 which is known as ROR gammaT.

  • Tested applications

    Suitable for: IHC-P, WB, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse thymus tissue.

  • General notes

    Ab232516 is the carrier-free version of ab207082. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232516 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Flow Cytometry - Anti-ROR gamma antibody [EPR20006] - BSA and Azide free (ab232516)
    Flow Cytometry - Anti-ROR gamma antibody [EPR20006] - BSA and Azide free (ab232516)

    Flow cytometric analysis of 4% paraformaldehyde-fixed Mouse thymocytes labeling ROR gamma with ab207082 at 1/50 dilution. Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    Note: Cell surface staining with PE conjugated anti-mouse CD8 and Alexa Fluor® 647 conjugated anti mouse CD4 antibodies, followed by fixation with 4% PFA for 10 min and intracellular staining with ab207082. The image shows thymocytes in the CD4+CD8+ (red) or the CD4+CD8- (black, negative control) gate. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207082).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR gamma antibody [EPR20006] - BSA and Azide free (ab232516)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR gamma antibody [EPR20006] - BSA and Azide free (ab232516)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling ROR gamma with ab207082 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on the cortical region of mouse thymus shown at a high magnification (400×) (PMID:14691482).

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207082).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR gamma antibody [EPR20006] - BSA and Azide free (ab232516)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR gamma antibody [EPR20006] - BSA and Azide free (ab232516)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling ROR gamma with ab207082 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadic nuclear staining on mouse spleen (PMID:14691482).

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207082).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-ROR gamma antibody [EPR20006] - BSA and Azide free (ab232516)
    Immunoprecipitation - Anti-ROR gamma antibody [EPR20006] - BSA and Azide free (ab232516)

    ROR gamma was immunoprecipitated from 0.35 mg of Mouse thymus lysate with ab207082 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab207082 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse thymus lysate, 10 μg (Input).

    Lane 2: ab207082 IP in Mouse thymus lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207082 inMouse thymus lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207082).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR gamma antibody [EPR20006] - BSA and Azide free (ab232516)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR gamma antibody [EPR20006] - BSA and Azide free (ab232516)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling ROR gamma with ab207082 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on mouse thymus cortex, located in the left and right of the image, with sporadic nuclear staining on medulla, middle region (PMID:14691482). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207082).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-ROR gamma antibody [EPR20006] - BSA and Azide free (ab232516)
    Anti-ROR gamma antibody [EPR20006] - BSA and Azide free (ab232516)

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