Human Anti-Cytomegalovirus IgG ELISA Kit (CMV) (ab108639)
Key features and details
- Sample type: Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Overview
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Product name
Human Anti-Cytomegalovirus IgG ELISA Kit (CMV) -
Detection method
Colorimetric -
Sample type
Serum -
Assay type
Indirect -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s anti-Cytomegalovirus (CMV) IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Cytomegalovirus in Human serum.
A 96-well plate has been precoated with Cytomegalovirus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Cytomegalovirus antigens. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Cytomegalovirus IgG sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution 1 x 50ml Cytomegalovirus (IgG) Coated Microplate (12 x 8 wells) 1 unit Cytomegalovirus anti-IgG HRP Conjugate 1 x 12ml Cytomegalovirus IgG Negative Control Blue cap 1 x 100µl Cytomegalovirus IgG Positive Control Purple cap 1 x 100µl Cytomegalovirus IgG Standard 0 - 0 U/mL White cap 1 x 100µl Cytomegalovirus IgG Standard 1 - 1.2 U/mL Yellow cap 1 x 100µl Cytomegalovirus IgG Standard 2 - 6 U/mL Red cap 1 x 100µl Cytomegalovirus IgG Standard 3 - 18 U/mL Green cap 1 x 100µl IgG Sample Diluent 1 x 22ml Stop Solution 1 x 11ml TMB Reagent 1 x 11ml -
Research areas
Images
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Typical Standard Curve
Representative standard curve using ab108639
