Goat Anti-Mouse IgG H&L (FITC) preadsorbed (ab7064)
Key features and details
- Goat Anti-Mouse IgG H&L (FITC) preadsorbed
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA
Overview
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Product name
Goat Anti-Mouse IgG H&L (FITC) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Mouse -
Tested applications
Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details -
Immunogen
Full length native Mouse IgG (purified).
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Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) preadsorbed (ab7064) Humphries, W.H. 4th et al PLoS One. 2011;6(10):e26626. doi: 10.1371/journal.pone.0026626. Epub 2011 Oct 24 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Rab7 and LAMP1 are highly colocalized in BS-C-1 cells and in HeLa cells
LAMP1 was detected with a mouse antiLAMP1 antibody in ICC/IF analysis of BS-C-1 cells.
Anti-LAMP1 was visualized using ab7064 at 1/500 diulution.
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Flow Cytometry - Goat Anti-Mouse IgG H&L (FITC) preadsorbed (ab7064)Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (FITC, pre-adsorbed) (ab7064) was used at 1/1000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.01μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. -
Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) preadsorbed (ab7064) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (FITC) preadsorbed (ab7064)
