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Goat Anti-Mouse IgG H&L (FITC) (ab6785)

Key features and details

  • Goat Anti-Mouse IgG H&L (FITC)
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC (PFA fixed), Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA, WB

Overview

  • Product name

    Goat Anti-Mouse IgG H&L (FITC)
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Tested applications

    Suitable for: IHC (PFA fixed), Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA, WBmore details

  • Immunogen

    mouse whole molecule

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • Conjugation notes

    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles FITC per mole of Goat IgG

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) (ab6785)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) (ab6785) This image is courtesy of an Abreview submitted by J Chai.

    ab8978 staining vimentin in human colon fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized with 0.1% Triton X-100 and blocked with Dako serum free protein blocker for 20 minutes at 28°C. Samples were incubated with primary antibody (1/100 in Dako antibody diluent) for 2 hours at 28°C. A FITC-conjugated goat anti-mouse IgG H&L (ab6785) (1/800) was used as the secondary antibody.

    See Abreview

  • Flow Cytometry - Goat Anti-Mouse IgG H&L (FITC) (ab6785)
    Flow Cytometry - Goat Anti-Mouse IgG H&L (FITC) (ab6785)
    Ab6785 was used at dilution 1/100 with the primary antibody ab52455 in Flow Cyt. See the review on ab52455.
  • Immunocytochemistry - Goat Anti-Mouse IgG H&L (FITC) (ab6785)
    Immunocytochemistry - Goat Anti-Mouse IgG H&L (FITC) (ab6785)
    Ab6785 was used at dilution 1/2000 with the primary antibody ab1220 in ICC/IF. See the review on ab1220.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (FITC) (ab6785)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (FITC) (ab6785)
    Ab6785 was used at dilution 1/300 with the primary antibody ab6002 in IHC-P. See the review on ab6002.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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