Anti-BioID2 antibody [SS 3A5-E2] (ab232733)
![Anti-BioID2 antibody [SS 3A5-E2] (ab232733)](https://www.abcam.com/ps/products/232/ab232733/Images/ab232733-353194-ab232733AP4443307GAPDHpublish.jpg "Anti-BioID2 antibody [SS 3A5-E2] (ab232733)")
Key features and details
- Mouse monoclonal [SS 3A5-E2] to BioID2
- Suitable for: ICC/IF, WB
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-BioID2 antibody [SS 3A5-E2]
See all BioID2 primary antibodies -
Description
Mouse monoclonal [SS 3A5-E2] to BioID2 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Fusion protein corresponding to BioID2. (Glutathione-S-transferase fused to A. aeolicus BioID2 (BPL R40G)).
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Positive control
- ICC/IF: HeLa-BioID2-Dox cells. WB: BiolD2-transfected HeLa whole cell lysate
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General notes
BioID2 is a second generation biotin protein ligase (BPL) that can be used to identify protein-protein interactions. BioID2 is derived from the hyperthermophilic bacterium Aquifex aeolicus and was mutated within the conserved biotin binding site (R40G) causing promiscuous protein biotinylation. BioID2 is the smallest known BPL which improves targeting of the bait protein and improves labelling of other proteins in proximity (PubMed ID 26912792).
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
SS 3A5-E2 -
Isotype
IgG1 -
Light chain type
kappa
Images
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Western blot - Anti-BioID2 antibody [SS 3A5-E2] (ab232733)All lanes : Anti-BioID2 antibody [SS 3A5-E2] (ab232733) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : BiolD2-TorsinA transfected HeLa whole cell lysate, non-induced
Lane 3 : BiolD2-TorsinA transfected HeLa whole cell lysate, DOX induced
Lysates/proteins at 10 µg per lane.
Performed under reducing conditions.
Observed band size: 70 kDa why is the actual band size different from the predicted?This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 3% milk before ab232733 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1ug/ml concentration and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Cell samples kindly provided by the Brian Burke laboratory, A-Star Institute, Singapore.
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![Immunocytochemistry/ Immunofluorescence - Anti-BioID2 antibody [SS 3A5-E2] (ab232733)](https://www.abcam.com/ps/products/232/ab232733/Images/ab232733-333746-anti-bioid2-antibody-ss-3a5-e2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BioID2 antibody [SS 3A5-E2] (ab232733)ab232733 staining BioID2 in HeLa cells expressing DOX-inducible BioID2 dystonia mutant of TorsinA. Parental HeLa cells have been used as negative control. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab232733 at 1 μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Cell samples kindly provided by the Brian Burke laboratory, A-Star Institute, Singapore.
