Anti-BirA antibody [6C4c7] (ab232732)
![Anti-BirA antibody [6C4c7] (ab232732)](https://www.abcam.com/ps/products/232/ab232732/Images/ab232732-381164-antibira6c4c7icchek293transfecteddox.jpg "Anti-BirA antibody [6C4c7] (ab232732)")
Key features and details
- Mouse monoclonal [6C4c7] to BirA
- Suitable for: ICC/IF, WB
- Reacts with: Escherichia coli
- Isotype: IgG1
Overview
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Product name
Anti-BirA antibody [6C4c7]
See all BirA primary antibodies -
Description
Mouse monoclonal [6C4c7] to BirA -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Recombinant fragment (GST-tag) within Escherichia coli BirA aa 1-150 (N terminal). The exact sequence is proprietary. Glutathione-S-transferase fused to E.coli BirA R118G
Database link: P06709 -
Positive control
- WB: BirA-transfected HEK293T whole cell lysates (induced). ICC: BirA transfected HEK293T (induced) cells
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
6C4c7 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-BirA antibody [6C4c7] (ab232732)
ab232732 staining BirA in normal HEK293 cells, BirA transfected HEK293T cells (uninduced) and BirA transfected HEK293T cells induced with DOX. The cells were fixed with 100% MeOH (5min), permeabilized with 0.1%PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab232732 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Cell samples kindly provided by the Brian Burke laboratory, A-Star Institute, Singapore.
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![Western blot - Anti-BirA antibody [6C4c7] (ab232732)](https://www.abcam.com/ps/products/232/ab232732/Images/ab232732-353191-ab232732AP47773435pcMilkPublish.jpg)
Western blot - Anti-BirA antibody [6C4c7] (ab232732)All lanes : Anti-BirA antibody [6C4c7] (ab232732) at 1 µg/ml
Lane 1 : HEK293 whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : BirA-SLAP75 transfected HEK293T whole cell lysate, mock induced
Lane 4 : BirA-SLAP75 transfected HEK293T whole cell lysate, DOX induced
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 5% milk before ab232732 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1µg/ml concentration and 1/20,000 dilution respectively. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
Cell samples kindly provided by the Brian Burke laboratory, A-Star Institute, Singapore.
