Recombinant E. coli BirA protein (Active) (ab135015)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 65% SDS-PAGE
- Active: Yes
- Tags: His-DDDDK tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
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Product name
Recombinant E. coli BirA protein (Active)
See all BirA proteins and peptides -
Biological activity
ab135015 (10 µg/ml) completely biotinylated 10 µM of substrate MBP-Avi at 30°C for 30 minutes in the standard reaction buffer containing 50 mM Bicine pH 8.3, 10 mM ATP, 10 mM MgOAc, and 50 µM d-Biotin. -
Purity
>= 65 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Escherichia coli -
Sequence
KDNTVPLKL IALLANGEFH SGEQLGETLG MSRAAINKHI QTLRDWGVDV FTVPGKGYSL PEPIQLLNAK QILGQLDGGS VAVLPVIDST NQYLLDRIGE LKSGDACIAE YQQAGRGRRG RKWFSPFGAN LYLSMFWRLE QGPAAAIGLS LVIGIVMAEV LRKLGADKVR VKWPNDLYLQ DRKLAGILVE LTGKTGDAAQ IVIGAGINMA MRRVEESVVN QGWITLQEAG INLDRNTLAA MLIRELRAAL ELFEQEGLAP YLSRWEKLDN FINRPVKLII GDKEIFGISR GIDKQGALLL EQDGIIKPWM GGEISLRSAE K -
Predicted molecular weight
37 kDa including tags -
Amino acids
2 to 321 -
Tags
His-DDDDK tag N-Terminus -
Additional sequence information
NP_418404
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 1.02% Imidazole
Constituents: 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.79% Tris HCl, 5% Glycerol (glycerin, glycerine), 0.29% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant E. coli BirA protein (ab135015)10% SDS-PAGE analysis of ab135015 (4 µg) stained with Coomassie Blue.
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Western blot - Recombinant E. coli BirA protein (ab135015)MBP-Avi biotinylation with His-FLAG-BirA using the standard reaction conditions as outlined in Enzyme Activity.
A. Coomassie blue stained gel showing
1. unbiotinylated MBP-Avi
2. His-FLAG-BirA / MBPAvi
3. MBP-Avi-biotin control
B. The corresponding Western blot probed with Streptavidin-HRP and developed with DAB (3,3’-Diaminobenzidine) substrate.
