Anti-BirA antibody [6C4c7] - BSA and Azide free (ab269579)
![Anti-BirA antibody [6C4c7] - BSA and Azide free (ab269579)](https://www.abcam.com/ps/products/269/ab269579/Images/ab269579-381334-antibira6c4c7icchek293transfecteddox.jpg "Anti-BirA antibody [6C4c7] - BSA and Azide free (ab269579)")
Key features and details
- Mouse monoclonal [6C4c7] to BirA - BSA and Azide free
- Suitable for: WB, ICC/IF
- Reacts with: Escherichia coli
- Isotype: IgG1
Overview
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Product name
Anti-BirA antibody [6C4c7] - BSA and Azide free
See all BirA primary antibodies -
Description
Mouse monoclonal [6C4c7] to BirA - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Recombinant fragment (GST-tag) within Escherichia coli BirA aa 1-150 (N terminal). The exact sequence is proprietary. Glutathione-S-transferase fused to E.coli BirA R118G
Database link: P06709 -
Positive control
- WB: BirA-transfected HEK293T whole cell lysates (induced). ICC: BirA transfected HEK293T (induced) cells
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab269579 is the carrier-free version of ab232732. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
6C4c7 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-BirA antibody [6C4c7] - BSA and Azide free (ab269579)
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab232732)
ab232732 staining BirA in normal HEK293 cells, BirA transfected HEK293T cells (uninduced) and BirA transfected HEK293T cells induced with DOX. The cells were fixed with 100% MeOH (5min), permeabilized with 0.1%PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab232732 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Cell samples kindly provided by the Brian Burke laboratory, A-Star Institute, Singapore.
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![Western blot - Anti-BirA antibody [6C4c7] - BSA and Azide free (ab269579)](https://www.abcam.com/ps/products/269/ab269579/Images/ab269579-368356-AntiBirA-antibody-6C4c7-BSA-Azide-free--western-blot-BirAtransfected-HEK293T-induced.jpg)
Western blot - Anti-BirA antibody [6C4c7] - BSA and Azide free (ab269579)All lanes : Anti-BirA antibody [6C4c7] (ab232732) at 1 µg/ml
Lane 1 : HEK293 whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : BirA-SLAP75 transfected HEK293T whole cell lysate, mock induced
Lane 4 : BirA-SLAP75 transfected HEK293T whole cell lysate, DOX induced
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 35 kDaThis blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 5% milk before ab232732 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1µg/ml concentration and 1/20,000 dilution respectively. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
Cell samples kindly provided by the Brian Burke laboratory, A-Star Institute, Singapore.
This image was produced using the same antibody clone but in a different formulation ab232732, PBS and sodium azide.
