Anti-Guanylyl Cyclase alpha 1/GUCY1A3 antibody (ab85445)
Key features and details
- Rabbit polyclonal to Guanylyl Cyclase alpha 1/GUCY1A3
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Guanylyl Cyclase alpha 1/GUCY1A3 antibody
See all Guanylyl Cyclase alpha 1/GUCY1A3 primary antibodies -
Description
Rabbit polyclonal to Guanylyl Cyclase alpha 1/GUCY1A3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Rat Guanylyl Cyclase alpha 1/GUCY1A3 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab97987) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Rat kidney; Rat lung; Mouse lung; Rat heart. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 5 µg/ml.IHC-P Use a concentration of 5 µg/ml.WB Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).Notes ICC/IF
Use a concentration of 5 µg/ml.IHC-P
Use a concentration of 5 µg/ml.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).Target
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Sequence similarities
Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
Contains 1 guanylate cyclase domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 2982 Human
- Entrez Gene: 60596 Mouse
- Entrez Gene: 497757 Rat
- Omim: 139396 Human
- SwissProt: Q02108 Human
- SwissProt: Q9ERL9 Mouse
- SwissProt: P19686 Rat
- Unigene: 24258 Human
see all -
Alternative names
- GC S alpha 1 antibody
- GC SA3 antibody
- GCS alpha 1 antibody
see all
Images
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Western blot - Anti-Guanylyl Cyclase alpha 1/GUCY1A3 antibody (ab85445)All lanes : Anti-Guanylyl Cyclase alpha 1/GUCY1A3 antibody (ab85445) at 1 µg/ml
Lane 1 : Kidney (Rat) Tissue Lysate
Lane 2 : Lung (Rat) Tissue Lysate
Lane 3 :Mouse lung normal tissue lysate - total protein (ab29297)
Lane 4 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 77 kDa
Observed band size: 77 kDa
Additional bands at: 48 kDa, 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Guanylyl Cyclase alpha 1/GUCY1A3 antibody (ab85445)IHC image of Guanylyl Cyclase alpha 1/GUCY1A3 staining in human Normal Kidney FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85445, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
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Immunocytochemistry/ Immunofluorescence - Anti-Guanylyl Cyclase alpha 1/GUCY1A3 antibody (ab85445)ICC/IF image of ab85445 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85445 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab85445 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Guanylyl Cyclase alpha 1/GUCY1A3 antibody (ab85445)All lanes : Anti-Guanylyl Cyclase alpha 1/GUCY1A3 antibody (ab85445) at 1 µg/ml
Lane 1 : Kidney (Rat) Tissue Lysate
Lane 2 : Lung (Rat) Tissue Lysate
Lane 3 :Mouse lung normal tissue lysate - total protein (ab29297)
Lane 4 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 77 kDa
Observed band size: 77 kDa
Additional bands at: 48 kDa, 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Guanylyl Cyclase alpha 1/GUCY1A3 antibody (ab85445)
IHC image of Guanylyl Cyclase alpha 1/GUCY1A3 staining in human Normal Kidney FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85445, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
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Immunocytochemistry/ Immunofluorescence - Anti-Guanylyl Cyclase alpha 1/GUCY1A3 antibody (ab85445)ICC/IF image of ab85445 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85445 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
