Anti-PDE5A/PDE5 antibody (ab224232)
Key features and details
- Rabbit polyclonal to PDE5A/PDE5
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PDE5A/PDE5 antibody
See all PDE5A/PDE5 primary antibodies -
Description
Rabbit polyclonal to PDE5A/PDE5 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog
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Immunogen
Recombinant fragment corresponding to Human PDE5A/PDE5 aa 300-450.
Database link: O76074 -
Positive control
- IHC-P: Human duodenum, pancreatic, and prostate tissue. ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE5A/PDE5 antibody (ab224232)
Paraffin-embedded human pancreas stained for PDE5A using ab224232 at 1/200 dilution in immunohistochemical analysis. Shows moderate cytoplasmic positivity in intercalacted ducts.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE5A/PDE5 antibody (ab224232)Paraffin-embedded human duodenum stained for PDE5A using ab224232 at 1/200 dilution in immunohistochemical analysis. Shows weak to moderate cytoplasmic positivity in glandular cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE5A/PDE5 antibody (ab224232)Paraffin-embedded human proostate stained for PDE5A using ab224232 at 1/200 dilution in immunohistochemical analysis. Shows weak to moderate cytoplasmic positivity in smooth muscle cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE5A/PDE5 antibody (ab224232)Paraffin-embedded human liver stained for PDE5A using ab224232 at 1/200 dilution in immunohistochemical analysis. Shows no positivity in hepatocytes as expected.
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Immunocytochemistry/ Immunofluorescence - Anti-PDE5A/PDE5 antibody (ab224232)PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for PDE5A/PDE5 (green) using ab224232 (4 µg/ml) in ICC/IF.
