Anti-PDE5A/PDE5 antibody (ab28761)
Key features and details
- Goat polyclonal to PDE5A/PDE5
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PDE5A/PDE5 antibody
See all PDE5A/PDE5 primary antibodies -
Description
Goat polyclonal to PDE5A/PDE5 -
Host species
Goat -
Specificity
This antibody is expected to recognise all 4 reported isoforms (as represented by NP_001074; NP_236914; NP_246273). [NP_237223 has been retracted]. -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Cow, Dog
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Immunogen
Synthetic peptide corresponding to Human PDE5A/PDE5 aa 864-875 (C terminal).
Sequence:C-LINGESG QAKRN
(Peptide available asab45684) -
Positive control
- Human lung lysate. Lysate prepared from transfected HEK293 cells transiently expressing PDE5A/PDE5 Flow Cyt (Intra): HEK293 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PDE5A/PDE5 antibody (ab28761)Anti-PDE5A/PDE5 antibody (ab28761) at 1 µg/ml + lysate prepared from transfected HEK293 cells transiently expressing PDE5A/PDE5 (isoform 2)
Predicted band size: 100 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
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Flow Cytometry - Anti-PDE5A/PDE5 antibody (ab28761)Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab28761 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-PDE5A/PDE5 antibody (ab28761)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation with ab28761 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
