Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PDE5A/PDE5 antibody (ab64179)

Key features and details

  • Rabbit polyclonal to PDE5A/PDE5
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-PDE5A/PDE5 antibody
    See all PDE5A/PDE5 primary antibodies

  • Description

    Rabbit polyclonal to PDE5A/PDE5

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Chicken, Dog, Chimpanzee

  • Immunogen

    Synthetic peptide corresponding to Human PDE5A/PDE5 aa 850 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab66454, ab66455)

  • Positive control

    • WB: MCF7 cells. ICC: MCF7 cells. IHC-P: Human heart tissue.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab64179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (4)
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
IHC-P
Use a concentration of 5 µg/ml.
ICC/IF (1)
Use a concentration of 1 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
IHC-P
Use a concentration of 5 µg/ml.
ICC/IF
Use a concentration of 1 µg/ml.

Target

  • Function

    Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.

  • Tissue specificity

    Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.

  • Pathway

    Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.

  • Sequence similarities

    Belongs to the cyclic nucleotide phosphodiesterase family.
    Contains 2 GAF domains.

  • Domain

    Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B.

  • Post-translational
    modifications

    Phosphorylation is regulated by binding of cGMP to the two allosteric sites.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 5''-cyclic phosphodiesterase antibody
    • CGB PDE antibody
    • CGB-PDE antibody
    • CGBPDE antibody
    • cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase antibody
    • cGMP binding cGMP specific 35 cyclic nucleotide phosphodiesterase antibody
    • cGMP binding cGMP specific phosphodiesterase antibody
    • cGMP binding/cGMP specific phosphodiesterase antibody
    • cGMP specific 3'5' cyclic phosphodiesterase antibody
    • cGMP specific phosphodiesterase PDE5A2 antibody
    • cGMP specific phosphodiesterase type 5A antibody
    • cGMP-binding cGMP-specific phosphodiesterase antibody
    • cGMP-specific 3'' antibody
    • CN5A antibody
    • CN5N antibody
    • PDE 5 antibody
    • PDE 5A antibody
    • PDE5 antibody
    • Pde5a antibody
    • PDE5A_HUMAN antibody
    • PDE5A1 antibody
    • Phosphodiesterase 5A antibody
    • Phosphodiesterase 5A cGMP specific antibody
    • Phosphodiesterase isozyme 5 antibody
    see all

Images

  • Western blot - Anti-PDE5A/PDE5 antibody (ab64179)
    Western blot - Anti-PDE5A/PDE5 antibody (ab64179)
    All lanes : Anti-PDE5A/PDE5 antibody (ab64179) at 1 µg/ml

    Lane 1 : Mouse skeletal muscle tissue lysate - total protein (ab29711)
    Lane 2 : Human ovary tissue lysate - total protein (ab30222)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 100 kDa
    Observed band size: 100 kDa
    Additional bands at: 42 kDa, 70 kDa. We are unsure as to the identity of these extra bands.

  • Immunocytochemistry/ Immunofluorescence - Anti-PDE5A/PDE5 antibody (ab64179)
    Immunocytochemistry/ Immunofluorescence - Anti-PDE5A/PDE5 antibody (ab64179)
    ICC/IF image of ab64179 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64179, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) MCF7 cells at 1µg/ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE5A/PDE5 antibody (ab64179)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE5A/PDE5 antibody (ab64179)

    IHC image of ab64179 staining PDE5A/PDE5 in Human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64179, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-PDE5A/PDE5 antibody (ab64179)
    Western blot - Anti-PDE5A/PDE5 antibody (ab64179)

References (7)

Publishing research using ab64179? Please let us know so that we can cite the reference in this datasheet.

ab64179 has been referenced in 7 publications.

  • Hitzerd E  et al. Placental effects and transfer of sildenafil in healthy and preeclamptic conditions. EBioMedicine 45:447-455 (2019). PubMed: 31204276
  • Ferrini MG  et al. Effect of ginger, Paullinia cupana, muira puama and l- citrulline, singly or in combination, on modulation of the inducible nitric oxide- NO-cGMP pathway in rat penile smooth muscle cells. Nitric Oxide 76:81-86 (2018). WB . PubMed: 29551532
  • Musicki B  et al. Testosterone replacement in transgenic sickle cell mice controls priapic activity and upregulates PDE5 expression and eNOS activity in the penis. Andrology 6:184-191 (2018). PubMed: 29145710
  • Schaffner D  et al. Analysis of the nitric oxide-cyclic guanosine monophosphate pathway in experimental liver cirrhosis suggests phosphodiesterase-5 as potential target to treat portal hypertension. World J Gastroenterol 24:4356-4368 (2018). PubMed: 30344420
  • Zhang N  et al. PDE5 Overexpression in Well-Differentiated Thyroid Carcinomas Is Associated with Lymph Node Metastasis. Int J Endocrinol 2017:6243932 (2017). PubMed: 29118812
  • Teich AF  et al. PDE5 Exists in Human Neurons and is a Viable Therapeutic Target for Neurologic Disease. J Alzheimers Dis 52:295-302 (2016). IHC . PubMed: 26967220
  • Zhang W  et al. Upregulation of Phosphodiesterase type 5 in the Hyperplastic Prostate. Sci Rep 5:17888 (2015). WB . PubMed: 26657792

Images

  • Western blot - Anti-PDE5A/PDE5 antibody (ab64179)
    Western blot - Anti-PDE5A/PDE5 antibody (ab64179)
    All lanes : Anti-PDE5A/PDE5 antibody (ab64179) at 1 µg/ml

    Lane 1 : Mouse skeletal muscle tissue lysate - total protein (ab29711)
    Lane 2 : Human ovary tissue lysate - total protein (ab30222)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 100 kDa
    Observed band size: 100 kDa
    Additional bands at: 42 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
  • Immunocytochemistry/ Immunofluorescence - Anti-PDE5A/PDE5 antibody (ab64179)
    Immunocytochemistry/ Immunofluorescence - Anti-PDE5A/PDE5 antibody (ab64179)
    ICC/IF image of ab64179 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64179, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) MCF7 cells at 1µg/ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE5A/PDE5 antibody (ab64179)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE5A/PDE5 antibody (ab64179)

    IHC image of ab64179 staining PDE5A/PDE5 in Human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64179, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-PDE5A/PDE5 antibody (ab64179)
    Western blot - Anti-PDE5A/PDE5 antibody (ab64179)

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