Anti-GAPex 5 antibody (ab86033)
Key features and details
- Rabbit polyclonal to GAPex 5
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GAPex 5 antibody -
Description
Rabbit polyclonal to GAPex 5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
A synthetic peptide corresponding to a region between residue 550 and 600 of human GAPex 5 (BAG10470.1).
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Positive control
- HeLa whole cell lysate (ab150035)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab86033 was affinity purified using an epitope specific to GAPex 5 immobilized on solid support -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GAPex 5 antibody (ab86033)All lanes : Anti-GAPex 5 antibody (ab86033) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 165 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 71 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
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Immunoprecipitation - Anti-GAPex 5 antibody (ab86033)ab86033, at 0.1 µg/ml, staining GAPex 5 in HeLa whole cell lysate immunoprecipitated using ab86033 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: by chemiluminescence with an exposure time of 30 seconds.