Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-MGST3 antibody [EPR12352] (ab192254)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12352] to MGST3
Suitable for: IHC-P, IP, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-MGST3 antibody [EPR12352]
See all MGST3 primary antibodies
Description

Rabbit monoclonal [EPR12352] to MGST3
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- A673, HepG2, Human fetal brain, and mouse spleen lysates; Human and mouse cardiac muscle and rat liver tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12352
Isotype

IgG
Research areas

Western blot - Anti-MGST3 antibody [EPR12352] (ab192254)

All lanes : Anti-MGST3 antibody [EPR12352] (ab192254) at 1/10000 dilution

Lane 1 : A673 cell lysate
Lane 2 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 17 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST3 antibody [EPR12352] (ab192254)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling MGST3 with ab192254 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
(inset: negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-MGST3 antibody [EPR12352] (ab192254)

Anti-MGST3 antibody [EPR12352] (ab192254) at 1/1000 dilution + Human fetal brain lysate at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 17 kDa
Western blot - Anti-MGST3 antibody [EPR12352] (ab192254)

Anti-MGST3 antibody [EPR12352] (ab192254) at 1/1000 dilution + mouse spleen lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 17 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST3 antibody [EPR12352] (ab192254)

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling MGST3 with ab192254 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
(inset: negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST3 antibody [EPR12352] (ab192254)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling MGST3 with ab192254 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
(inset: negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-MGST3 antibody [EPR12352] (ab192254)

Western blot analysis of immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab192254 at 1/20 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Anti-MGST3 antibody [EPR12352] (ab192254)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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