Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MGST3 antibody [EPR12352] (ab192254)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12352] to MGST3
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MGST3 antibody [EPR12352]
    See all MGST3 primary antibodies

  • Description

    Rabbit monoclonal [EPR12352] to MGST3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • A673, HepG2, Human fetal brain, and mouse spleen lysates; Human and mouse cardiac muscle and rat liver tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MGST3 antibody [EPR12352] (ab192254)
    Western blot - Anti-MGST3 antibody [EPR12352] (ab192254)
    All lanes : Anti-MGST3 antibody [EPR12352] (ab192254) at 1/10000 dilution

    Lane 1 : A673 cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST3 antibody [EPR12352] (ab192254)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST3 antibody [EPR12352] (ab192254)

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling MGST3 with ab192254 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
    (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-MGST3 antibody [EPR12352] (ab192254)
    Western blot - Anti-MGST3 antibody [EPR12352] (ab192254)
    Anti-MGST3 antibody [EPR12352] (ab192254) at 1/1000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 17 kDa

  • Western blot - Anti-MGST3 antibody [EPR12352] (ab192254)
    Western blot - Anti-MGST3 antibody [EPR12352] (ab192254)
    Anti-MGST3 antibody [EPR12352] (ab192254) at 1/1000 dilution + mouse spleen lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST3 antibody [EPR12352] (ab192254)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST3 antibody [EPR12352] (ab192254)

    Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling MGST3 with ab192254 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
    (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST3 antibody [EPR12352] (ab192254)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST3 antibody [EPR12352] (ab192254)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling MGST3 with ab192254 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
    (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-MGST3 antibody [EPR12352] (ab192254)
    Immunoprecipitation - Anti-MGST3 antibody [EPR12352] (ab192254)

    Western blot analysis of immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab192254 at 1/20 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

  • Anti-MGST3 antibody [EPR12352] (ab192254)
    Anti-MGST3 antibody [EPR12352] (ab192254)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-MGST3 antibody [EPR12352] (ab192254)

© 2021 Astana Biomed Group LLP. All rights reserved.