Recombinant E. coli BirA protein (ab198449)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 78% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Conjugation, Purification, SDS-PAGE
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Product name
Recombinant E. coli BirA protein
See all BirA proteins and peptides -
Biological activity
BirA (16 µg/mL) completely biotinlabels 10 µM of Avi-tag substrate at 30°C for 30 minutes in reaction buffer containing 50 mM Bicine pH 8.3, 10 mM ATP, 10 mM MgOAc, and 50 µM dBiotin.
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Purity
>= 78 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Escherichia coli -
Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLVPRGSKDNTVPLKLIALLANGEFHSGEQL GETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQ LDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKWFS PFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPN DLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWIT LQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRP VKLIIGDKEIFGISRGIDKQGALLLE QDGIIKPWMGGEISLRSAEK -
Predicted molecular weight
61 kDa including tags -
Amino acids
2 to 321 -
Tags
GST tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.49% GlutathioneThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Conjugation - Recombinant E. coli BirA protein (ab198449)
Image A: SDS-PAGE with Coomassie blue showing:
Lane 1: Unbiotinylated BirA
Lane 2: ab198449
Lane 3: BRD4-Avi-biotin control
Lane 4: Protein markers
Image B. Shows the corresponding Western blot probed with Streptavidin-HRP and developed with ECL reagent.
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SDS-PAGE - Recombinant E. coli BirA protein (ab198449)10% SDS-PAGE with Coomassie staining: 4 µg ab198449.
