Serum Antibody Purification Kit (Protein G) (ab128751)
Key features and details
- Sample type: Ascites Fluid, Serum
Overview
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Product name
Serum Antibody Purification Kit (Protein G)
See all Antibody Purification kits -
Sample type
Serum, Ascites Fluid -
Product overview
Protein G has a high affinity for the Fc regions of IgG molecules from a variety of species. ab128751 G Serum resin is prepared by coupling purified Protein G to agarose beads. It can therefore be used to purify IgG fractions from both serum and ascites fluid. The antibody is captured on the AbSelect G resin and unwanted substances are removed by a simple wash procedure. Up to 10mg of antibody can be purified in each run. The volume of sample required will depend on the host species.
The purified product is then eluted and neutralized.
The antibodies purified with Protein G Serum Antibody Purification Kit are fully compatible with our Lightning-Link® antibody conjugation kits and Oligonucleotide Conjugation Kit. To purify antibodies for use with our nanoparticle conjugation kits, please use Antibody Purification Kit - Nanoparticles (ab204909).
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Notes
This product is manufactured by Expedeon, an Abcam company, and was previously called AbSelect™ G Serum Antibody Purification System. 893-0030 is the same as the 1 test size. 893-0500 is the same as the 3 tests size.
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 tests 3 tests ab274299 - Elution Buffer 1 x 5ml 1 x 15ml ab275847 - G Binding Buffer (10X) 1 x 6ml 1 x 18ml ab274174 - Neutralizer Buffer 1 x 2ml 1 x 5ml ab274180 - Purification Column 1 unit 1 unit ab275849 - Serum Protein G Resin 1 x 4ml 3 x 4ml ab274173 - Wash Buffer 1 x 25ml 1 x 75ml -
Research areas
