TCS Antibody Purification Kit (ab109207)
Overview
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Product name
TCS Antibody Purification Kit
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Product overview
TCS Antibody Purification Kit (ab109207) has been designed for tissue culture supernatant purification, and can be used to purify 10-50 mL in each purification. Each column can purify up to 5 mg of antibody.
This kit works by coupling highly purified protein A to agarose beads and can therefore be used to purify IgG fractions from hybridoma supernatants. The antibody is captured on the TCS resin and unwanted substances are removed by a simple wash procedure. The purified product is then eluted and neutralized.
The antibodies purified with the TCS Antibody Purification Kit are fully compatible with our Lightnign-Link® Antibody Conjugation kits and our Oligonucleotide Conjugation Kit. To purify antibodies from TCS for use with our nanoparticle conjugation kits, please use our TCS Antibody Purification Kit - Nanoparticles (ab270554).
ab109207 is not suitable for goat antibody purification.
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Notes
This product is manufactured by Expedeon, an Abcam company, and was previously called AbSelect™ A Antibody Purification System. 862-0500 is the same as the 3 tests size. 862-0030 is the same as the 1 test size.
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 3 tests 1 tests ab274298 - Binding Buffer (10X) 1 x 18ml 1 x 6ml ab274299 - Elution Buffer 1 x 15ml 1 x 5ml ab274174 - Neutralizer Buffer 1 x 5ml 1 x 2ml ab273957 - Protein A resin 3 x 2ml 1 x 2ml ab274180 - Purification Column 3 units 1 unit ab273958 - Spin cartridge/ collecting tube assemblies 3 units 1 unit ab274173 - Wash Buffer 1 x 75ml 1 x 25ml -
Research areas