Choline Kinase Assay Kit (ab239729)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate, Purified protein, Tissue Lysate
Overview
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Product name
Choline Kinase Assay Kit -
Detection method
Fluorescent -
Sample type
Cell Lysate, Purified protein, Tissue Lysate -
Product overview
In Choline Kinase Assay Kit (ab239729), choline is phosphorylated by choline kinase producing a series of intermediates, which react with the fluorometric probe generating a strong flurorescence signal (Ex/Em = 535/587 nm). The signal is directly proportional to choline kinase activity present in the biological sample. The assay is fast, sensitive, reproducible and is suitable for measuring choline kinase activity in different cells and other biological samples This kit can detect as low as 0.5 μM of choline kinase activity.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 100 tests ADP Standard (1 µmole) 1 vial Assay Buffer 1 x 50ml ATP 1 vial Detection Mix I 1 vial Detection Mix II 1 vial Human ChoK 1 x 10µl Probe 1 x 0.4ml Substrate 1 vial -
Relevance
Choline kinase is an enzyme that catalyzes the first reaction in the CDP-choline pathway for the synthesis of phosphatidylcholine, a membrane phospholipid. This transferase, utilizes Mg2+ as a cofactor in order to transfer a phosphate moiety from ATP to choline molecules forming phosphocholine. In humans, there are two isozymes, Choline Kinase A and B. Both proteins share more than 60% in homology. Choline Kinase plays pivotal roles in different human diseases: elevated Choline kinase A expression has been found in breast, lung, colorectal, prostate and bladder tumor tissues whereas choline kinase B is suggested to be involved in muscular dystrophy.
Images
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ADP Standard Curve
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Kinetic reaction rate of ChoK with and without choline substrate in the assay. -
Activity of ChoK determined by the assay in different samples.