Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TIE2 antibody [33] (ab280905)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [33] to TIE2
  • Suitable for: Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-TIE2 antibody [33]
    See all TIE2 primary antibodies

  • Description

    Mouse monoclonal [33] to TIE2

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HUVEC lysates. Flow Cyt: HUVEC cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    33

  • Isotype

    IgG1

  • Light chain type

    kappa

Images

  • Western blot - Anti-TIE2 antibody [33] (ab280905)
    Western blot - Anti-TIE2 antibody [33] (ab280905)
    All lanes : Anti-TIE2 antibody [33] (ab280905) at 1/1000 dilution

    Lane 1 : HUVEC(human umbilical vein endothelial cell ), whole cell lysate
    Lane 2 : 293T ( human embryonic kidney epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 125 kDa
    Observed band size: 120,150 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight  observed is consistent with what has been described in the literature (PMID:Â 28396397)

    Negative control: HEK-293T (PMID: 17189382).

     

     Exposure time: 37 seconds

     

  • Flow Cytometry - Anti-TIE2 antibody [33] (ab280905)
    Flow Cytometry - Anti-TIE2 antibody [33] (ab280905)

    Flow cytometric analysis of HEK-293 (Human embryonic kidney epithelial cell, Left) / HUVEC (Human umbilical vein endothelial cell, Right) cells labelling TIE2 with ab280905 at 1/1000 dilution (0.1ug) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Mouse IgG (Alexa Fluor® 647, ab150119) at 1/2000 dilution was used as the secondary antibody. Negative control: HEK-293.

  • Anti-TIE2 antibody [33] (ab280905)
    Anti-TIE2 antibody [33] (ab280905)

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