Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µg 351 792 ₸ 10 µg 117 264 ₸

Order now and get it on Friday March 26, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [33] to TIE2
Suitable for: Flow Cyt, WB
Reacts with: Human

Product name

Anti-TIE2 antibody [33]
See all TIE2 primary antibodies
Description

Mouse monoclonal [33] to TIE2
Host species

Mouse
Tested applications

Suitable for: Flow Cyt, WBmore details
Unsuitable for: ICC or IHC-P
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HUVEC lysates. Flow Cyt: HUVEC cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

33
Isotype

IgG1
Light chain type

kappa

Western blot - Anti-TIE2 antibody [33] (ab280905)

All lanes : Anti-TIE2 antibody [33] (ab280905) at 1/1000 dilution

Lane 1 : HUVEC(human umbilical vein endothelial cell ), whole cell lysate
Lane 2 : 293T ( human embryonic kidney epithelial cell), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 125 kDa
Observed band size: 120,150 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:Â 28396397)

Negative control: HEK-293T (PMID: 17189382).

Exposure time: 37 seconds
Flow Cytometry - Anti-TIE2 antibody [33] (ab280905)

Flow cytometric analysis of HEK-293 (Human embryonic kidney epithelial cell, Left) / HUVEC (Human umbilical vein endothelial cell, Right) cells labelling TIE2 with ab280905 at 1/1000 dilution (0.1ug) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Mouse IgG (Alexa Fluor® 647, ab150119) at 1/2000 dilution was used as the secondary antibody. Negative control: HEK-293.
Anti-TIE2 antibody [33] (ab280905)

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