Alexa Fluor® 647 Anti-CD11c antibody [N418] (ab233046)
![Alexa Fluor® 647 Anti-CD11c antibody [N418] (ab233046)](https://www.abcam.com/ps/products/233/ab233046/Images/ab233046-1-ab233046FLOW1.jpg "Alexa Fluor® 647 Anti-CD11c antibody [N418] (ab233046)")
Key features and details
- Alexa Fluor® 647 Armenian hamster monoclonal [N418] to CD11c
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Isotype: IgG
Overview
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Product name
Alexa Fluor® 647 Anti-CD11c antibody [N418]
See all CD11c primary antibodies -
Description
Alexa Fluor® 647 Armenian hamster monoclonal [N418] to CD11c -
Host species
Armenian hamster -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse
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Immunogen
Tissue, cells or virus corresponding to Mouse CD11c.
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Positive control
- Flow Cyt: C57BL/6 mouse splenocytes.
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
N418 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Alexa Fluor® 647 Anti-CD11c antibody [N418] (ab233046)
C57BL/6 mouse splenocytes stained with ab233046 (right) or Armenian Hamster IgG Alexa Fluor® 647 (monoclonal) (left). C57BL/6 Mouse splenocytes were incubated for 30 min on ice in 1x PBS containing 10 µg/ml anti CD16/32 antibody and 10% normal goat serum to block FC receptors and non-specific protein-protein interactions followed by the antibody (ab233046) or Armenian Hamster IgG 647 Isotype (1x106 in 100 µl at 1:500 (1 µg/ml)) for 30 min on ice. The cells were simultaneously stained with the following conjugated antibodies: MHC Class II PerCP, CD19 APC and CD3 PE.
Acquisition of >50,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter. Gating strategy: T and B cells were excluded from viable lymphocytes and expression of MHC Class II and CD11c was assessed.
